Apheresis Column for Chemokine Receptor Cell Depletion
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Solution Overview
Problem
Current treatments for respiratory conditions like sarcoidosis and COPD are limited in effectively addressing inflammation, particularly in cases with permanently constricted airways and extensive lung damage, as they fail to adequately reduce the recruitment of inflammatory leukocytes to sites of inflammation.
Innovation Solution
The method involves using specific binding reagents, such as biotinylated chemokines immobilized on a solid support within an apheresis column, to capture and remove chemokine receptor-expressing cells, including CCR2, CCR1, CCR3, CCR5, CXCR1, and CCR7 expressing cells from peripheral blood, thereby reducing their recruitment to inflammatory sites.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If bronchodilator drugs are used to treat respiratory conditions, then breathing difficulties are addressed, but inflammation and recruitment of inflammatory leukocytes are not effectively reduced
Solution Approach 1:
The invention extracts and removes inflammatory leukocytes from the patient's blood using an apheresis column with specific binding reagents that target chemokine receptors on these cells, thereby separating the harmful inflammatory cells from the beneficial blood components and returning the purified blood to the patient
Solution Approach 2:
The invention uses chemokine binding reagents as intermediaries that specifically recognize and bind to chemokine receptors on inflammatory leukocytes, enabling selective capture and removal of these cells while leaving other blood components unaffected
2Object-affected harmful factors
If apheresis is used to remove blood components, then inflammatory cells can be depleted, but the treatment complexity and device requirements increase
Solution Approach 1:
The invention employs disposable apheresis columns pre-loaded with specific chemokine binding reagents, eliminating the need for complex reusable device sterilization and calibration systems, thereby reducing overall system complexity while maintaining effective leukocyte depletion
Solution Approach 2:
The apheresis column design integrates multiple functions including blood filtration, selective cell capture, and fluid return in a single device, reducing the need for multiple separate components and simplifying the overall treatment system
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively depletes specific chemokine receptor-expressing cells from the peripheral blood, potentially alleviating symptoms, preventing progression, and promoting regression or recovery in respiratory conditions by reducing inflammatory cell recruitment.
Implementation Method 1
specific binding reagents, such as biotinylated chemokines immobilized on a solid support within an apheresis column, to capture and deplete chemokine receptor-expressing cells
Data Source
AI summary
A method for treating a respiratory conditions comprises applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR2, CCR1, CCR3, CCR5, CXCR1, CXCR2 and/or CCR7 immobilized directly or indirectly on the support thus removing one or more chemokine receptor, optionally CCR2, CCR1, CCR3, CCR5, CXCR1, CXCR2 and/or CCR7 expressing cells from the peripheral blood of the patient or subject. Various companion diagnostic methods and useful binding reagents are also described.