Apoptosis-Induced Stem Cells for Cryoprotectant-Free Preservation
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Solution Overview
Problem
Existing stem cell therapeutic agents are sensitive to environmental conditions, have a short shelf life, and conventional cryoprotectants like DMSO cause cytotoxicity and immune responses, making them unsuitable for long-term preservation and administration.
Innovation Solution
Cryopreservation of stem cells without cryoprotectants using a blood substitute solution, inducing apoptosis to increase IL-10 expression, resulting in apoptosis-induced cells with enhanced therapeutic potential for inflammatory and renal diseases.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If conventional cryoprotectants like DMSO are used for stem cell preservation, then cells can be preserved for long periods, but the cells suffer from cytotoxicity and immune responses
Solution Approach 1:
The invention extracts and removes harmful cryoprotectants (DMSO, FBS) from the preservation system, replacing them with a safe alternative composition that maintains cell viability without causing cytotoxicity or immune responses
Solution Approach 2:
The invention introduces an intermediary preservation composition consisting of serum-free culture medium supplemented with insulin and hydrocortisone, which mediates between the need for long-term preservation and the requirement to avoid cytotoxicity
2Reliability
If cryoprotectants containing animal-derived components like FBS are used, then cells can be preserved effectively, but there is a risk of viral infections and immune responses
Solution Approach 1:
The invention extracts and eliminates animal-derived components (fetal bovine serum, human serum) from the preservation system, replacing them with a synthetic serum-free composition that eliminates viral and prion contamination risks
Solution Approach 2:
The invention changes the composition parameters from animal-derived serum components to a defined serum-free medium with specific concentrations of insulin (10^-6 M) and hydrocortisone (10^-6 M), transforming an unreliable natural product into a controlled synthetic system
3Duration of action of stationary object
If stem cells are cryopreserved using conventional methods, then long-term storage is achieved, but cell characteristics are compromised
Solution Approach 1:
The invention introduces an intermediary preservation composition that maintains cell characteristics during long-term storage, using serum-free medium with insulin and hydrocortisone to preserve cellular integrity without the damaging effects of conventional cryoprotectants
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apoptosis-induced stem cells exhibit a 1.5-fold increase in IL-10 expression, providing effective prevention and treatment of inflammatory and renal diseases without cytotoxicity, maintaining therapeutic efficacy post-thawing.
Implementation Method 1
cryopreservation of stem cells without cryoprotectants using a blood substitute solution
Data Source
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AI summary
The present disclosure relates to apoptosis-induced stem cells, a method for producing the same, and a pharmaceutical composition for the prevention or treatment of an inflammatory disease or a renal disease, including the same. Cells according to an aspect and a composition including the cells as an active ingredient have an increased IL-10 expression level without including a cryoprotectant, and thus can be effectively used for the prevention or treatment of an inflammatory disease or a renal disease.