AprL-clade Subtilisin Variants for Detergent Stability
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Solution Overview
Problem
There is a need for engineered proteases that are suitable for specific conditions and uses, as existing serine proteases do not adequately address certain industrial and cleaning applications.
Innovation Solution
Development of AprL-clade variant subtilisin enzymes with specific amino acid modifications that enhance stability and soil removal performance compared to the parent AprL-clade subtilisin enzyme.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing serine proteases are used, then general proteolytic activity is provided, but stability and soil removal performance for specific industrial applications are insufficient
Solution Approach 1:
The patent applies local quality by introducing specific amino acid modifications at particular positions (e.g., S258P, T210P, G127T, A128P) to enhance stability and soil removal performance in specific regions of the enzyme molecule, while maintaining overall proteolytic activity. This targeted modification approach allows the enzyme to achieve improved reliability for specific industrial applications without compromising its general functionality.
Solution Approach 2:
The patent employs parameter changes by systematically varying amino acid sequences through site-directed mutagenesis and selecting variants with optimized properties. Specific amino acid substitutions (e.g., S258P, T210P, G127T, A128P) are introduced to modify enzyme characteristics such as stability, thermostability, and soil removal performance, thereby adapting the enzyme to specific industrial conditions while maintaining catalytic activity.
2Reliability
If amino acid modifications are introduced to improve stability, then enzyme reliability increases, but enzyme complexity increases
Solution Approach 1:
The patent introduces amino acid modifications at specific local positions (e.g., S258P, T210P, G127T, A128P) rather than throughout the entire enzyme structure. This localized approach enhances detergent stability and reliability while minimizing increases in overall structural complexity, as only specific regions of the enzyme are modified to achieve the desired performance improvement.
Solution Approach 2:
The patent applies partial action by introducing a limited number of specific amino acid modifications rather than comprehensive restructuring of the enzyme. This approach achieves sufficient improvement in detergent stability and reliability through targeted changes at key positions, avoiding the complexity that would result from more extensive structural modifications.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The AprL-clade variant subtilisin enzymes demonstrate improved stability and cleaning performance, achieving higher performance indices in various protease activity assays and detergent stability tests.
Implementation Method 1
Serine proteases are enzymes (EC No. 3.4.21) possessing an active site serine that initiates hydrolysis of peptide bonds of proteins
Data Source
AI summary
The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.