Fluorescent Aptamer Conjugates for Pathogen Surface Detection
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Solution Overview
Problem
Current methods for detecting pathogens on surfaces are inefficient and lack specificity, often requiring complex equipment and procedures.
Innovation Solution
A method involving the application of a liquid composition containing aptamer conjugates, which are specifically bound to pathogens, followed by illumination and fluorescence detection, allowing for the visualization of pathogens on surfaces.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional pathogen detection methods are used, then detection can be performed, but the methods are inefficient and lack specificity
Solution Approach 1:
The invention changes the detection parameter from conventional non-specific staining to specific fluorescent aptamer binding. The aptamers are selected to specifically bind to pathogen surfaces, and fluorescence parameters are used to detect this specific binding, thereby improving both specificity and efficiency simultaneously
Solution Approach 2:
The invention replaces complex mechanical detection equipment with a simple fluorescence visualization system. Instead of using complex instruments for pathogen detection, the patent uses fluorescent aptamers that can be visualized with simple light sources and filters, improving efficiency while maintaining high specificity
2Reliability
If conventional detection equipment is used, then pathogen detection is possible, but complex equipment and procedures are required
Solution Approach 1:
The aptamers perform self-service by specifically binding to pathogen surfaces and carrying their own fluorescent labels. The aptamers automatically locate and bind to target pathogens without requiring complex equipment, and the fluorescent signal self-indicates the presence of pathogens, simplifying the overall detection system while maintaining reliability
Solution Approach 2:
The invention uses fluorescence color changes as a simple visual indicator of pathogen presence. The fluorescent aptamers emit specific wavelengths of light when bound to pathogens, allowing detection through simple optical filtering and visualization without complex equipment, thereby reducing device complexity while maintaining detection accuracy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables efficient and specific visualization of pathogens on surfaces, facilitating rapid detection and potential decontamination, thereby improving surface hygiene and safety.
Implementation Method 1
one or more fluorophore, wherein the one or more fluorophore absorbs the emitted light at the one or more predetermined wavelength range and emits light at a longer wavelength range
Implementation Method 2
a quencher to quench nonspecific fluorescence
Implementation Method 3
the one or more aptamer conjugate has a specific binding affinity for one or more target molecule of the one or more pathogens
Data Source
AI summary
Methods and systems of detecting the presence, absence, or amount of target molecule in a sample or on a surface are provided.