Solid Support Storage for Aptamer Stability
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Solution Overview
Problem
Short nucleic acid sequences, such as aptamers, face stability issues during storage and handling, which complicates their use in analyte detection and SELEX processes, requiring a method for long-term stabilization at ambient temperature.
Innovation Solution
The use of solid supports like cellulose-based papers or fibrous materials for storing short single-stranded nucleic acids, which allows for prolonged ambient temperature storage and subsequent amplification for analyte detection, utilizing Rolling Circle Amplification (RCA) or other amplification methods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If short nucleic acid sequences are stored in solution at ambient temperature, then ease of operation is improved, but stability deteriorates
Solution Approach 1:
The patent introduces a solid support matrix as an intermediary carrier for aptamer storage. The matrix provides a stable environment that protects nucleic acids from degradation while maintaining ambient temperature storage conditions, thus resolving the contradiction between ease of operation and stability.
Solution Approach 2:
The patent changes the physical state parameter of aptamer storage from liquid solution to solid support-bound format. This parameter change fundamentally alters the stability characteristics, allowing long-term storage at ambient temperature without the degradation issues associated with solution-phase storage.
2Stability of the object's composition
If cold storage is used to maintain aptamer stability, then stability is improved, but device complexity and operational requirements worsen
Solution Approach 1:
The patent changes the storage temperature parameter from refrigerated/cold conditions to ambient temperature by simultaneously changing the physical state from solution to solid support-bound format. This eliminates the need for complex cold storage infrastructure while maintaining aptamer stability.
3Stability of the object's composition
If short nucleic acids are stored on solid supports, then stability is improved, but retrieval and amplification efficiency worsen
Solution Approach 1:
The patent performs preliminary action by designing the solid support system to facilitate easy elution of aptamers prior to amplification. The support matrix is chosen to allow efficient release of nucleic acids when needed, thus maintaining high amplification efficiency despite the stable bound storage state.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively stabilizes aptamers and microRNA on solid supports, enabling reliable storage and amplification, facilitating efficient analyte detection and SELEX processes without the need for cold storage, and allows for the generation of significant amounts of specific aptamers for multiplex assays.
Implementation Method 1
storing short single stranded nucleic acids (10-100 nt), such as aptamers and micro RNA, on a solid support
Implementation Method 2
Rolling circle amplification (RCA) is an isothermal DNA amplification process mediated by DNA polymerases in which ssDNA is synthesised via circular templates
Data Source
AI summary
The present invention relates to a method and kit for analyte detection. More precisely the invention relates to a method of detecting an analyte, comprising storing short single stranded nucleic acids (10-100 nt), such as aptamers and micro RNA, on a solid support at ambient temperature; and subsequent amplification of said nucleic acids for detection of analyte(s).