Aptamer-Coated Transduction Bags Without Manual Enhancer Coating
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Solution Overview
Problem
Existing transduction methods for introducing foreign DNA into target cells, such as hematopoietic cells and suspension cells, are inefficient and require manual coating of enhancers like retronectin, which are costly and time-consuming, and existing technologies negatively impact cell viability.
Innovation Solution
A container, such as a bag, comprising a fluoropolymer with functional groups and aptamer sequences that bind to viral vectors and cell receptors, facilitating efficient transduction without manual coating.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If manual coating of enhancers like retronectin is performed, then transduction efficiency is improved, but cost and time consumption increase
Solution Approach 1:
The aptamer sequences are pre-coated onto the container surface during manufacturing, eliminating the need for manual coating before use. This preliminary action resolves the technical contradiction by embedding the enhancer function into the container itself, saving time and resources while maintaining transduction efficiency.
Solution Approach 2:
The container is designed to provide the enhancer function autonomously through integrated aptamer sequences on its inner surface. The container serves itself by incorporating the coating function directly into its structure, eliminating the need for separate manual coating steps and reducing overall process complexity.
2Reliability
If manual coating of enhancers like retronectin is performed, then transduction efficiency is improved, but cost increases
Solution Approach 1:
The aptamer sequences are applied to the container inner surface during the manufacturing process rather than requiring separate manual coating steps. This preliminary action reduces manufacturing complexity and cost while ensuring consistent enhancer function for improved transduction efficiency.
Solution Approach 2:
The container structure and enhancer function are merged into a single integrated component. The aptamer sequences are incorporated directly into the container material or applied during container formation, combining the container and enhancer into one element that reduces overall system complexity and cost.
3Reliability
If conventional enhancers like polybrene or protamine sulfate are used, then transduction efficiency is improved, but cell viability is negatively impacted
Solution Approach 1:
The invention changes the chemical and biological parameters of the enhancer by using aptamer sequences instead of conventional small molecules like polybrene or protamine sulfate. This parameter change maintains transduction efficiency while improving cell viability, as aptamers provide a more biocompatible enhancement mechanism.
Solution Approach 2:
The aptamer sequences act as a biological intermediary that mediates between the viral vector and target cell without the harmful effects of conventional enhancers. The aptamers facilitate viral vector binding and cellular uptake through specific molecular interactions that are more compatible with cell viability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The container enhances transduction efficiency by co-localizing viral vectors and target cells, reducing the need for manual coating and maintaining cell viability.
Implementation Method 1
a first aptamer sequence having a binding affinity for a viral vector
Data Source
AI summary
This disclosure relates generally to containers (such as bags) having surfaces comprising one or more aptamer sequences. More particularly, the present disclosure relates to containers such as bags comprising a fluoropolymer attached to aptamer sequences having binding affinity for one or more biological agents, and to transductions methods using such containers. In one aspect, the disclosure provides a container (e.g., a bag) having an outer surface and an inner surface, the inner surface comprising a fluoropolymer; attached to the fluoropolymer, a plurality of functional groups; and attached to each of at least a portion of the functional groups, a first aptamer sequence having a binding affinity for a viral vector.