Aptasensor Detecting Female Blood via 17β-Estradiol Fluorescence
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Solution Overview
Problem
Current methods for detecting female-specific blood traces at crime scenes are inefficient, requiring large analysis efforts and resources due to mixed samples, and existing antibody-based techniques are costly, time-consuming, and limited in application.
Innovation Solution
Development of an aptasensor using aptamers that emit light when bound to 17β-estradiol, a female-specific hormone, allowing for efficient gender discrimination at crime scenes through a portable detection apparatus.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If antibody-based detection methods are used, then sensitivity is improved, but production cost and time increase significantly
Solution Approach 1:
The patent replaces expensive, time-consuming antibody production with disposable aptamer-based sensors. Aptamers are synthesized chemically rather than through biological production, making them cheaper and faster to produce while maintaining detection functionality.
Solution Approach 2:
The patent substitutes biological antibody-antigen recognition systems with chemical aptamer-target molecular recognition. This replacement eliminates the need for cell culture and animal-based antibody production, significantly reducing time and cost while achieving comparable or superior sensitivity.
2Measurement precision
If antibody-based detection methods are used, then sensitivity is improved, but production cost increases
Solution Approach 1:
The patent employs disposable aptamer sensors that are chemically synthesized rather than biologically produced. This approach dramatically reduces production costs by eliminating expensive cell culture media, animal housing, and purification facilities while maintaining high detection sensitivity.
Solution Approach 2:
The patent replaces the complex biological antibody production system with a simpler chemical aptamer synthesis system. This substitution eliminates numerous expensive production steps including immunization, cell culture, and protein purification, resulting in significant cost reduction.
3Reliability
If mixed blood samples are collected for gene identification, then detection coverage is improved, but analysis manpower and expense increase
Solution Approach 1:
The patent extracts and identifies female-specific blood traces from mixed samples using aptamer-based detection before full gene identification. This preliminary sorting step separates female samples from male samples, allowing focused analysis only on relevant samples and significantly improving overall analysis efficiency.
Solution Approach 2:
The patent performs preliminary gender identification using aptamer sensors before conducting expensive and time-consuming gene identification procedures. This preliminary action filters samples to identify only those requiring full analysis, reducing overall manpower and expense while maintaining comprehensive detection coverage.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The aptasensor effectively distinguishes female-specific blood traces using a portable variable light source, reducing analysis manpower and expenses while maintaining stability and specificity, enabling accurate gender identification.
Implementation Method 1
an aptasensor for detecting a female-specific trace of blood including an aptamer which is used as a biosensor and emits light as the aptamer is bound to the female-specific trace of blood
Data Source
AI summary
The present invention relates to an aptasensor for detecting a female-specific trace of blood including an aptamer which is used as a biosensor and emits light as the aptamer is bound to the female-specific trace of blood, and a detection apparatus using the same. The aptasensor for detecting a trace of blood to discriminate genders from the trace of blood at a crime scene has a configuration in which the aptasensor emits light as the aptasensor is binding to the female-specific trace of blood, so that genders of a suspect and a victim is distinguished from each other at the crime scene.