Aqueous Solution Chelating Agent Membrane Filterability
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Solution Overview
Problem
Current methods for preparing aqueous solutions for cell culture face challenges in achieving excellent membrane filterability, which is crucial for producing biopharmaceuticals like glycoproteins and antibodies, due to high production costs and limitations in equipment and scale-up, despite efforts to enhance components and conditions.
Innovation Solution
Adding a chelating agent, such as citric acid, malic acid, or ethylenediaminetetraacetic acid, to the aqueous solution prior to final pH adjustment improves membrane filterability by enhancing the concentration-dependent filtration process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If membrane area is increased to improve membrane filterability, then filtration efficiency is improved, but costs and facility restrictions increase
Solution Approach 1:
The patent changes the chemical composition parameters of the aqueous solution by adding chelating agents (EDTA, citric acid, malic acid) at specific concentrations (0.01-10 mM) to improve membrane filterability without increasing membrane area, thereby resolving the contradiction between filtration efficiency and manufacturing cost
2Manufacturing precision
If dissolution conditions are improved to enhance membrane filterability, then filtration performance is improved, but remarkable improvement is not achieved
Solution Approach 1:
The patent introduces chelating agents as intermediary substances that mediate between the aqueous solution components and the membrane filter. These chelating agents bind to metal ions and other components that cause membrane clogging, thereby significantly improving filtration efficiency beyond what dissolution condition adjustments alone can achieve
3Productivity
If productivity is improved by enhancing components and addition or enrichment of new components, then production efficiency is improved, but membrane filterability becomes more difficult to achieve
Solution Approach 1:
The patent applies preliminary action by adding chelating agents to the aqueous solution before filtration and cell culture processes. This pre-treatment prevents membrane clogging and maintains solution stability throughout subsequent high-productivity operations, allowing productivity enhancement without compromising filterability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly improves membrane filterability, allowing for more efficient production of physiologically active substances by stabilizing the aqueous solution and facilitating faster filtration during cell culture processes.
Implementation Method 1
Chelating agents such as citric acid, malic acid, ethylenediaminetetraacetic acid or the like have been widely known as one of the components contained in the aqueous solution for cell culture
Data Source
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AI summary
Provided are a method for preparing a highly versatile aqueous solution having remarkably improved membrane filterability, which can be stably membrane-filtered in a short time, an aqueous solution prepared by the preparation method, a method for culturing cells using the aqueous solution which is prepared by the preparation method, a method for producing a physiologically active substance using the culturing method, a physiologically active substance produced by the production method of the aqueous solution, a method for performing membrane filtration of the aqueous solution which is prepared by the preparation method of the aqueous solution, a method for improving membrane filterability of the aqueous solution, and a method for producing the physiologically active substance by preparing the aqueous solution, performing membrane filtration of the aqueous solution, and then culturing cells using the resulting aqueous solution. The present invention relates to a method for preparing an aqueous solution, characterized by addition of a chelating agent.