Polypeptide and Gene Markers for Arteriosclerosis Diagnosis
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Solution Overview
Problem
Current methods for diagnosing arteriosclerosis lack sufficient accuracy and specificity, necessitating the development of more effective polypeptide and gene markers for early detection of arteriosclerotic lesions.
Innovation Solution
Identification and utilization of novel polypeptide markers and gene markers, along with specific antibodies and DNA probes, for detecting arteriosclerosis through DNA microarrays and kits that enable precise identification of arteriosclerosis-related proteins and genes in serum samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional biochemical examination methods (LDL, CRP, chlamydia antibody) are used for arteriosclerosis diagnosis, then the diagnostic process is simple and widely available, but the detection accuracy and specificity are insufficient
Solution Approach 1:
The invention segments the diagnostic approach by identifying and measuring multiple specific polypeptide markers (including apolipoprotein A-I, apolipoprotein B-100, and other lipoprotein-associated polypeptides) individually or in combination. This segmentation allows for more precise detection of arteriosclerosis by analyzing specific molecular components rather than using general biochemical markers, thereby improving measurement precision while maintaining a manageable diagnostic framework through focused marker selection.
2Reliability
If early stage detection of arteriosclerosis is pursued, then the diagnostic value increases, but the difficulty of detecting and measuring markers increases
Solution Approach 1:
The invention employs parameter changes by utilizing multiple different polypeptide markers and their various forms (including oxidized forms, complexed forms, and different isoforms) to detect arteriosclerosis at early stages. By measuring parameters such as the concentration ratios of different polypeptides, their oxidation states, and their complex formation with other molecules, the method enhances diagnostic reliability for early detection while managing the complexity through targeted measurement of specific molecular parameters rather than attempting to detect all possible early-stage changes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The proposed solution significantly enhances the accuracy of arteriosclerosis detection, allowing for early identification of arteriosclerotic lesions and unstable plaques, improving diagnostic capabilities.
Implementation Method 1
an antibody which specifically binds to the polypeptide as an antigen
Implementation Method 2
a probe for detecting an arteriosclerosis marker gene
Data Source
Figure 1(a)~1(e)
AI summary
Disclosed are: a polypeptide marker for diagnosing arteriosclerosis; a gene marker for diagnosing arteriosclerosis; an antibody; a probe for detecting an arteriosclerosis marker gene; a DNA microarray or a DNA chip for detecting an arteriosclerosis marker gene; a method for detecting arteriosclerosis; and a kit for diagnosing arteriosclerosis; with which an arteriosclerotic lesion can be detected with much improved accuracy. Specifically disclosed are: a polypeptide marker for diagnosing arteriosclerosis, which comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO: 31 of the Sequence Listing, or a partial amino acid sequence thereof; a gene which encodes the amino acid sequence; a probe for detecting the gene; a DNA microarray or a DNA chip comprising the probe; an antibody bindable to the polypeptide as an antigen; a kit comprising any one of the above-mentioned items; and a method for detecting arteriosclerosis by using any one of the above-mentioned items.