Ascorbic Acid Oxidase Stabilization via Nitrous Acid Coexistence
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Solution Overview
Problem
Ascorbic acid oxidase is unstable and deactivates during the preservation of clinical reagents, leading to deteriorated performance in biological sample measurements due to its susceptibility to interfering substances.
Innovation Solution
Coexisting ascorbic acid oxidase with nitrous acid or its salt/ester in an aqueous medium stabilizes the enzyme, maintaining its activity for long-term preservation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ascorbic acid oxidase is added to suppress ascorbic acid interference, then measurement accuracy is improved, but enzyme stability deteriorates during preservation
Solution Approach 1:
Sodium nitrite acts as an intermediary stabilizing agent that mediates between the ascorbic acid oxidase and the preservation environment. It forms a protective interaction with the enzyme, preventing deactivation during storage while allowing the enzyme to function normally during measurement.
Solution Approach 2:
The invention changes the chemical environment by introducing sodium nitrite, which alters the preservation conditions. This parameter change (adding nitrite) transforms the unstable preservation state into a stable one, maintaining enzyme activity over time without affecting measurement accuracy.
2Ease of operation
If ascorbic acid oxidase is preserved in solution state, then ease of operation is improved, but enzyme deactivation accelerates
Solution Approach 1:
Sodium nitrite is added in advance to the solution before preservation begins. This preliminary action prepares the protective environment, preventing enzyme deactivation during the entire preservation period while maintaining the convenient solution state for later use.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method ensures that the ascorbic acid oxidase activity remains at 60% or more after 10 days of preservation at 30°C, effectively stabilizing the enzyme and enhancing the reliability of clinical tests.
Implementation Method 1
an ascorbic acid oxidase is stably maintained by allowing the ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium
Data Source
AI summary
Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.