Aseptic Sampling Apparatus Pressure Gradient Control
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Solution Overview
Problem
Current aseptic sampling methods for cell cultures are complex, costly, and require extensive manual sterilization processes, making it difficult to maintain aseptic conditions and efficiently sample culture liquids for analysis.
Innovation Solution
An aseptic sampling apparatus with a sampling channel and pressure applying device that maintains a pressure gradient of 1 kPa/m or more within the sampling channel, preventing contamination by ensuring a sufficient fluid pressure to expel any invading germs and eliminating the need for manual sterilization of the nozzle.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If manual sterilization processes are used before and after sampling, then aseptic conditions can be maintained, but the sampling process becomes complex and time-consuming
Solution Approach 1:
The sampling system performs self-sterilization by maintaining continuous positive pressure in the sampling line, which prevents contamination without requiring external sterilization operations. The system uses its own pressure control mechanism to maintain aseptic conditions throughout the sampling process.
Solution Approach 2:
The patent replaces manual sterilization operations with a pressure-based protective mechanism. Instead of using steam, flame, or chemical sterilization, the system uses controlled positive pressure to prevent contamination, eliminating complex sterilization equipment and procedures.
2Reliability
If multiple sterilization and washing steps are performed, then contamination is prevented, but the sampling interval cannot be shortened
Solution Approach 1:
The sampling line maintains continuous positive pressure throughout the sampling process, providing continuous protection against contamination. This eliminates the need for discrete sterilization steps between samples, enabling continuous or near-continuous sampling operations.
Solution Approach 2:
The system establishes positive pressure in the sampling line before sampling begins and maintains it throughout the process. This preliminary pressure establishment prevents contamination from the outset, eliminating the need for post-sampling sterilization and washing steps.
3Device complexity
If a simple sampling configuration is used, then cost is reduced, but contamination risk increases
Solution Approach 1:
The patent uses pneumatic pressure control to maintain positive pressure in the sampling line. This simple yet effective approach uses gas pressure to create a protective barrier against contamination without requiring complex mechanical or chemical sterilization systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus allows for continuous, contamination-free sampling and analysis of cell cultures with a simple and cost-effective configuration, reducing the risk of contamination and minimizing the amount of culture liquid used for analysis.
Implementation Method 1
the pressure applying device controls a flow pressure or a liquid flow speed of a fluid in the sampling channel so that a pressure gradient toward an open-end nozzle on an outlet side of the sampling channel is 1 kPa/m or more
Data Source
AI summary
The aseptic sampling apparatus according to the present invention includes: a sampling channel for pulling out a culture solution in a culture tank to supply the same to an analytical unit; and a pressure-applying unit for applying a pressure to the inside of the sampling channel. The pressure-applying unit controls the flow pressure or fluid flow rate of a fluid in the sampling channel such that a pressure gradient toward the outlet-side open end nozzle of the sampling channel is not less than 1 kPa/m.