ASG5 Apocrine Cell Line for Long-Term Proliferation
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Solution Overview
Problem
There is a lack of a human-derived apocrine cell line that exhibits long-term proliferation, which hampers research on apocrine gland functions, particularly in understanding sweating mechanisms and developing deodorants, as existing cell lines lose morphological and functional characteristics within a few passages.
Innovation Solution
A human apocrine cell line, ASG5, is developed and maintained through at least 30 subcultures, allowing for indefinite proliferation, and characterized using electrophysiological, molecular, and biochemical techniques to mimic the functions of apocrine glands, including steroid synthesis and odor precursor analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of moving object
If primary apocrine cell lines are cultured, then apocrine gland function can be studied, but the cells lose morphological and functional characteristics after a few passages
Solution Approach 1:
The patent applies parameter changes by optimizing culture conditions including specific growth factors (EGF, bFGF), serum concentration (5-10% FCS), and passage timing to maintain apocrine cell characteristics throughout extended culture periods, allowing long-term studies without loss of functional properties
Solution Approach 2:
The patent creates a stable cell line model that copies the in vivo apocrine gland architecture and function, enabling researchers to study authentic apocrine physiology and pathology in vitro without requiring continuous primary cell isolation, thus preserving cell characteristics indefinitely
2Loss of information
If in vivo studies are used to study apocrine gland functions, then comprehensive data can be obtained, but ethical concerns and complexity increase
Solution Approach 1:
The patent introduces a cell line model as an intermediary system that bridges in vivo and in vitro research, capturing the complexity of living apocrine glands while providing a simplified, controllable in vitro platform for ethical and practical research applications
Data Source
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Figure 4A~4B
AI summary
An Apocrine cell line An apocrine cell line is obtained by the steps of isolating an apocrine cell from primary tissue, culturing the isolated cell in a first culture medium, removing unattached cells from the first culture medium and transferring said unattached cells to a second culture medium comprising an effective concentration of a phorbol ester, and thereby establishing an apocrine cell line exhibiting long-term proliferation capability, which after many cultures is indicative of indefinite proliferation.