L-Aspartate Oxidase Expression for Higher-Yield Stress-Resilient Plants

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Solution Overview

Problem

Existing methods for increasing plant biomass, seed yield, and stress resistance do not comprehensively address issues related to biomass production, abiotic stress resistance, biotic stress resistance, germination regulation, and seed yield, and some methods, such as using neonicotinoid insecticides, have environmental concerns.

Innovation Solution

Overexpressing the L-aspartate oxidase enzyme in plants to increase NAD and derivatives, enhancing photosynthetic capacities, growth rates, and stress resistance, thereby improving biomass, seed yield, and germination rates.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If neonicotinoid insecticides are used to increase yields, then biotic stress resistance is improved, but environmental harm increases due to bee mortality

Engineering Contradiction:

Improvebiotic stress resistanceVSAvoidenvironmental harm

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effect of neonicotinoid insecticides on bees into a beneficial approach by using genetic engineering to enhance plant immunity naturally. Instead of relying on chemical insecticides that harm pollinators, the invention uses modified RNA interference pathways to provide biotic stress resistance through biological mechanisms, thereby eliminating the need for harmful chemicals while maintaining or improving pest resistance

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent replaces the mechanical/chemical system of insecticide application with a biological/genetic system. By modifying RNA interference pathways and enhancing endogenous immunity, the plant develops intrinsic resistance to biotic stresses, substituting chemical control methods with genetically programmed biological defense mechanisms

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If conventional methods are used to increase biomass and seed yield, then productivity is improved, but comprehensive stress resistance and germination regulation are not addressed

Engineering Contradiction:

Improvebiomass and seed yieldVSAvoidcomprehensive stress resistance

Core Design Contradiction:

ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent applies multi-functionality by simultaneously enhancing multiple plant traits through modification of RNA interference pathways. The genetic modifications provide comprehensive benefits including increased biomass, improved seed yield, enhanced abiotic stress resistance, improved biotic stress resistance, and better germination regulation, allowing a single intervention to address multiple agricultural challenges

Inventive Principle:

Principle #6Universality (Multi-functionality)

3Productivity

If photosynthetic pathways are genetically manipulated to increase biomass, then productivity is improved, but energy balance and stress resistance may be compromised

Engineering Contradiction:

Improvebiomass productionVSAvoidstress resistance

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies parameter changes by modifying RNA interference pathway parameters rather than directly manipulating photosynthetic pathways. This indirect approach adjusts gene expression patterns and metabolic regulation in a way that enhances biomass production while maintaining or improving stress resistance, avoiding the trade-offs associated with direct photosynthetic engineering

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The overexpression of L-aspartate oxidase leads to significant increases in NAD content, resulting in accelerated growth, enhanced seed yield, improved abiotic and biotic stress resistance, and reduced need for phytosanitary treatments.

Implementation Method 1

The method according to the invention enables an increase in the photosynthetic capacities of the plants via an increase in the amounts of NAD and derivatives thereof in said plants

Methodology Applied

Scientific EffectOxidation: Oxidation

Data Source

PatentUS12480134B2Plants with increased yield and method for producing said plants

Publication Date: 2025.11.25 UNIV PARIS SACLAY

AI summary

The invention relates to a method for increasing the yield and biomass of a plant, by means of an increase in the expression of the L-aspartate oxidase in the plant. The method according to the invention allows an increase in the photosynthetic capacities of the plants as a result of an increase in the quantities of NAO and the derivatives thereof in said plants. The invention relates to the plants produced by such a method.