Genetically Modified Microorganism Aspartic Acid Yield
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for producing aspartic acid by microbial fermentation face challenges in achieving high yield and low cost, while also minimizing the production of by-products such as other amino acids and organic acids.
Innovation Solution
A genetically modified microorganism with reduced or inactivated citrate synthase and oxaloacetate decarboxylase activities, along with modifications to other enzymes such as succinate dehydrogenase, lactate dehydrogenase, phosphoenolpyruvate carboxylase, and pyruvate: quinone oxidoreductase, is used to enhance aspartic acid production and reduce by-product formation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microbial fermentation is used to produce aspartic acid, then production can be achieved, but the yield is low and production cost is high
Solution Approach 1:
The invention changes the metabolic parameters of the microorganism by deleting specific genes (citrate synthase gene, oxaloacetate decarboxylase gene, and other related genes) to alter the metabolic flux distribution. This genetic modification redirects carbon flow toward aspartic acid production, significantly improving yield from glucose while reducing by-product formation, thereby lowering production costs
Solution Approach 2:
The invention extracts or removes specific metabolic pathways by deleting genes responsible for competing reactions (citrate synthase, oxaloacetate decarboxylase, succinate dehydrogenase, etc.). By taking out these alternative pathways, the metabolic flux is concentrated toward aspartic acid production, improving efficiency and reducing by-products
2Quantity of substance
If conventional fermentation is used, then aspartic acid can be produced, but by-products such as other amino acids and organic acids are generated in high amounts
Solution Approach 1:
The invention removes competing metabolic pathways by deleting genes for citrate synthase, oxaloacetate decarboxylase, succinate dehydrogenase, fumarate reductase, lactate dehydrogenase, and pyruvate: quinone oxidoreductase. This extraction of alternative pathways prevents the formation of by-products while concentrating metabolic flux toward aspartic acid
Solution Approach 2:
The invention changes metabolic parameters by introducing modified phosphoenolpyruvate carboxylase with reduced feedback inhibition by aspartic acid. This parameter change allows continuous carbon flow toward aspartic acid production without being blocked by product accumulation, while simultaneously preventing by-product formation through gene deletions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The genetically modified microorganism significantly improves the production amount and yield of aspartic acid while reducing the production of by-products, thereby lowering production costs and increasing efficiency.
Implementation Method 1
The production of aspartic acid by a biotechnological method is mainly performed by a bioreactor using immobilized bacterial cells of Escherichia coli having aspartase activity, from fumaric acid as a raw material.
Implementation Method 2
oxaloacetate decarboxylase activity is reduced or inactivated
Data Source
AI summary
A genetically modified microorganism which satisfies at least one condition selected from the group consisting of the following conditions (I) and (II). Condition (I): citrate synthase activity is reduced or inactivated compared with a wild-type microorganism corresponding to the genetically modified microorganism, and condition (II): oxaloacetate decarboxylase activity is reduced or inactivated compared with the wild-type microorganism.