Aspergillus sp. DH4 Aerobic Denitrification with Zero-Valent Iron
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Solution Overview
Problem
Current biological nitrogen removal technologies face challenges in efficiently removing nitrogen from water bodies, particularly due to the lack of carbon sources for traditional heterotrophic denitrification and the need for improved aerobic denitrification processes.
Innovation Solution
The use of Aspergillus sp. DH4, enhanced by zero-valent iron as an inorganic electron donor, for aerobic denitrification in nitrogen-containing water bodies, thereby improving nitrogen removal efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If traditional heterotrophic denitrification is used, then nitrogen removal can be achieved, but carbon sources are insufficient due to low C/N ratio in wastewater
Solution Approach 1:
The patent changes the electron donor type from organic carbon to inorganic electron donors (H2, Fe2+, S2O3 2-), fundamentally altering the denitrification pathway from heterotrophic to autotrophic or mixotrophic, thereby resolving the carbon source limitation in low C/N ratio wastewater
Solution Approach 2:
The Aspergillus sp. DH4 strain is engineered to perform multiple functions: aerobic respiration, anaerobic respiration (denitrification), and utilization of multiple inorganic electron donors (H2, Fe2+, S2O3 2-), making it adaptable to various wastewater conditions with different carbon availability
2Productivity
If aerobic denitrification is implemented, then simultaneous nitrification and denitrification can occur, but denitrification efficiency is insufficient without adequate electron donors
Solution Approach 1:
The patent introduces inorganic electron donors (H2, Fe2+, S2O3 2-) as alternative electron sources, changing the metabolic pathway from organic carbon-dependent to inorganic electron donor-dependent denitrification, enabling simultaneous nitrification and denitrification under aerobic conditions
Solution Approach 2:
The inorganic electron donors act as intermediaries that bridge the gap between aerobic conditions and denitrification requirements, providing electrons for nitrate reduction while allowing oxygen to remain present in the system
3Productivity
If inorganic electron donors are added to enhance denitrification, then nitrogen removal efficiency improves, but process complexity and cost increase
Solution Approach 1:
The system utilizes naturally occurring or easily added inorganic substances (H2 from water decomposition, Fe2+ from common salts, S2O3 2- from sodium thiosulfate) that can be introduced through simple dosing mechanisms, avoiding complex infrastructure while achieving enhanced nitrogen removal
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Aspergillus sp. DH4 with zero-valent iron enhances nitrate removal rates, achieving complete nitrogen removal within six days at optimal conditions, while minimizing ammonia accumulation.
Implementation Method 1
enhanced by zero-valent iron as an inorganic electron donor
Implementation Method 2
The Aspergillus sp. DH4 with aerobic denitrification enhanced by the inorganic electron donor
Data Source
AI summary
An Aspergillus sp. DH4 with aerobic denitrification enhanced by an inorganic electron donor and its application are provided, which relates to the field of microbial technologies. The Aspergillus sp. DH4 is preserved at China Center for Type Culture Collection (CCTCC), a preservation address is Wuhan University, 299 Bayi Road, Wuchang District, Wuhan City, China, a preservation number is CCTCC NO: M20232690, and a preservation date is Dec. 27, 2023.