Aspergillus niger Peptidase for Feed Inhibitor Hydrolysis
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Solution Overview
Problem
Current animal feed compositions containing cereals, pulses, and tubers often include anti-nutritional factors like alpha-amylase/trypsin inhibitors, which hinder optimal growth performance and lead to intestinal inflammation and excessive nitrogen excretion in monogastric animals.
Innovation Solution
Incorporation of Aspergillus niger aspergilloglutamic peptidase into animal feed to efficiently hydrolyze these inhibitors, improving protein digestibility and reducing intestinal inflammation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If cereals, pulses, oilseeds, and tubers are included in animal feed to provide proteins, then protein supply is improved, but anti-nutritional factors like alpha-amylase/trypsin inhibitors cause reduced digestibility and intestinal inflammation
Solution Approach 1:
The feed composition includes exogenous proteases that act preliminarily on anti-nutritional factors before they can harm the animal. These enzymes pre-digest the inhibitors in the feed, converting harmful proteins into harmless peptides and amino acids before ingestion, thereby eliminating the harmful effects while preserving nutritional value
Solution Approach 2:
The invention converts harmful anti-nutritional factors into beneficial components through enzymatic hydrolysis. The proteases transform the harmful inhibitor proteins into useful peptides and amino acids that can be readily absorbed by the animal, turning a detrimental substance into a nutritional benefit
2Stability of the object's composition
If protease inhibitors are present in plant storage organs to protect proteins, then protein stability is improved, but animal growth performance and digestibility deteriorate
Solution Approach 1:
Exogenous proteases are added to the feed to preliminarily hydrolyze protease inhibitors before the animal consumes the feed. This preliminary action occurs during feed storage or preparation, allowing the inhibitors to be broken down into non-inhibitory peptides before they can interfere with endogenous proteases in the animal's digestive system
Solution Approach 2:
The invention changes the molecular structure of protease inhibitors through enzymatic hydrolysis, breaking down the intact inhibitor proteins into smaller peptide fragments. This parameter change in molecular size and structure eliminates the inhibitory activity while preserving or enhancing nutritional value through the release of amino acids
3Device complexity
If feed contains anti-nutritional factors, then feed composition complexity is reduced, but nitrogen excretion increases and environmental impact worsens
Solution Approach 1:
Proteases are added to the feed to preliminarily digest anti-nutritional factors and improve protein breakdown before ingestion. This preliminary enzymatic action increases the availability of amino acids for absorption, thereby reducing the amount of nitrogen that would otherwise be excreted as waste products
Solution Approach 2:
The invention converts the potentially harmful effect of anti-nutritional factors that lead to nitrogen waste into a beneficial process. By using proteases to break down these factors, the system transforms what would be wasted nitrogen into absorbable amino acids, thereby reducing environmental nitrogen excretion while improving feed efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of Aspergillus niger aspergilloglutamic peptidase enhances animal performance by improving feed conversion ratio, daily weight gain, and reducing nitrogen excretion while minimizing intestinal inflammation.
Implementation Method 1
the Aspergillus niger aspergilloglutamic peptidase is capable of efficiently hydrolyzing alpha-amylase/trypsin inhibitors that are present in wheat, barley and related cereal species, as well as hydrolyzing trypsin inhibitors that are present in pulses, oilseeds, and tubers
Data Source
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AI summary
This invention relates to the use of the Aspergillus niger aspergilloglutamic peptidase in a feed composition containing cereals, pulses, oilseeds, and/or tubers to improve animal performance, reduce nitrogen excretion and/or reduce intestinal inflammation. Furthermore, the present invention relates to a feed composition or a feed additive comprising the Aspergillus niger aspergilloglutamic peptidase. The present invention relates to the discovery that the Aspergillus niger aspergilloglutamic peptidase is capable of efficiently hydrolyzing alpha- amylase/trypsin inhibitors that are present in wheat, barley and related cereal species, as well as hydrolyzing trypsin inhibitors that are present in pulses, oilseeds, and tubers. Furthermore, the present invention relates to a method of improving animal performance comprising improving feed conversion ratio, and/or improving daily weight gain, and/or reducing intestinal inflammation, and/or reducing nitrogen excretion in an animal fed a feed stuff containing alpha- amylase and/or trypsin inhibitors, comprising orally administering a sufficient amount of the Aspergillus niger aspergilloglutamic peptidase. It also relates to the pretreatment of feedstuff with the Aspergillus niger aspergilloglutamic peptidase.