Automated Assay Apparatus for Multi-Well Plate Analysis
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Solution Overview
Problem
Current methods for conducting assays in multi-well plate formats lack improved apparatuses, methods, and kits for automated sampling, sample preparation, and analysis, particularly for luminescence assays.
Innovation Solution
A kit comprising a multi-well assay test plate with auxiliary plates containing dry assay reagents, along with an apparatus for supporting and translating the test plate, and a pipettor subassembly for delivering samples and reagents, enabling efficient sample and reagent handling and analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual sample preparation and handling is used, then device complexity is reduced, but productivity and throughput are limited
Solution Approach 1:
The system uses automated robotic arms and mechanical handlers to perform sample preparation, plate handling, and reagent dispensing without manual intervention. The apparatus autonomously navigates, positions plates, and executes assay protocols, enabling high-throughput operation while reducing the need for complex manual操作流程
Solution Approach 2:
The assay system is designed to handle multiple plate formats (96-well, 384-well, 1536-well) and perform various assay types (luminescence, fluorescence, absorbance) using the same automated apparatus. The robotic system can adapt to different plate configurations and assay protocols, achieving high productivity without proportionally increasing device complexity
2Productivity
If automated sample preparation and analysis is implemented, then productivity is improved, but device complexity increases
Solution Approach 1:
The automated system is divided into functional modules: plate handlers for different plate formats, robotic arms for sample and reagent transfer, detection systems for multiple assay types, and control software for protocol management. This modular segmentation allows high automation capability while managing complexity through independent, specialized components
Solution Approach 2:
The apparatus employs dynamic, reconfigurable robotic arms and mechanical handlers that can adapt their movements and configurations based on the specific plate format and assay protocol. The system dynamically adjusts its operation to match the requirements of different assay types, achieving high productivity without requiring separate dedicated equipment for each assay format
3Measurement precision
If conventional assay methods are used, then ease of operation is maintained, but sensitivity and measurement precision are limited
Solution Approach 1:
The system replaces manual mechanical operations with automated robotic handlers and electronic control systems. Sample preparation, plate handling, and data acquisition are performed by automated mechanisms rather than manual techniques, enhancing measurement precision through consistent, repeatable operations while minimizing user intervention to program initiation and result interpretation
Solution Approach 2:
The automated assay system incorporates real-time monitoring and feedback control during sample preparation and measurement. The system automatically adjusts parameters based on detected signals and protocol requirements, ensuring optimal sensitivity and precision without requiring users to manually optimize each measurement condition
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enhances sensitivity, throughput, and multiplexing capabilities, allowing for automated random access analysis with reduced consumable requirements and minimal user intervention.
Implementation Method 1
apparatuses, methods, and kits for conducting automated sampling, sample preparation, and/or sample analysis in a multi-well plate assay format
Data Source
AI summary
We describe apparatuses, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of liquid samples in a clinical point of care setting.