Assay Interference Reduction via Spatial Segmentation

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing bio/chemical sensing and testing methods face challenges in reducing the interference effects from elements in samples, which hinder accurate detection and measurement of analytes due to interference from physical and biochemical effects.

Innovation Solution

The method involves aggregating interference elements into specific regions within the sample, allowing for the identification and measurement of analyte signals in interference-free areas, using an imager and software to distinguish between interference-rich and interference-poor regions, and compressing the sample into a thin layer to enhance signal detection.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If traditional sensing methods are used to detect analytes in samples, then the detection process is simple, but interference elements in the sample cause inaccurate measurements and reduce measurement precision

Engineering Contradiction:

Improveanalyte detection accuracyVSAvoidinterference from sample elements

Core Design Contradiction:

Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The sample is divided into multiple regions, with interference elements segregated into specific zones and analyte detection performed in separate interference-free zones. This spatial segmentation allows simultaneous presence of interference elements and accurate analyte measurement without mutual interference.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

Interference elements are extracted or removed from the sample matrix through various mechanisms (such as binding to specific substrates or being sequestered in designated regions), separating them from the analyte detection zone. This extraction eliminates the harmful interference while preserving analyte integrity.

Inventive Principle:

Principle #2Taking out (Extraction)

2Measurement precision

If aggregation reagents are added to concentrate interference elements, then interference reduction is improved, but the device complexity and ease of operation deteriorate

Engineering Contradiction:

Improveinterference reduction effectivenessVSAvoidassay procedure complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The assay system is designed to automatically perform interference element aggregation and segregation without requiring manual intervention. The device autonomously manages the complex steps of adding aggregation reagents, incubating samples, and analyzing results, thereby maintaining high measurement precision while simplifying user operation.

Inventive Principle:

Principle #25Self-service

Solution Approach 2:

The assay device is designed with multi-functionality to handle various sample types and interference scenarios using a standardized protocol. The same device can process different analytes and accommodate different interference element types, reducing the need for multiple specialized procedures and simplifying overall operation.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentUS20200333322A1Assays with reduced interference

Publication Date: 2020.10.22 ESSENLIX CORP

AI summary

The present invention provides devices, systems, and methods, for performing biological and chemical assays.