Assay Article Solid Surface Binding Member Immobilization
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Solution Overview
Problem
Conventional target assay methods require separate chambers for the second binding member, leading to complex device structures and potential instability of the second binding member due to high dilution, which affects the accuracy and stability of target detection.
Innovation Solution
A method involving a solid surface with distinct regions for immobilizing a first binding member, a blocking material, and a second binding member labeled with an enzyme, where the first and second binding members bind to different sites of the target, eliminating the need for a separate chamber for the second binding member and enhancing stability through higher concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a separate chamber is used for the second binding member, then the device structure becomes complex, but the second binding member stability improves
Solution Approach 1:
The patent merges the second binding member with the solid surface by directly immobilizing it onto the solid support, eliminating the need for a separate chamber. This integration simplifies the device structure while maintaining the binding member's functionality through proper immobilization techniques.
Solution Approach 2:
The invention extracts the second binding member from the solution phase and immobilizes it directly onto the solid surface. This extraction from solution eliminates the dilution issue that causes instability, while the solid-phase immobilization provides the necessary stability without requiring a separate chamber.
2Device complexity
If the second binding member is kept in solution, then the device structure remains simple, but the binding capability decreases due to high dilution
Solution Approach 1:
The second binding member is preliminarily immobilized onto the solid surface before the assay is performed. This preliminary immobilization concentrates the binding member at the detection site, ensuring sufficient binding capability and detection accuracy without requiring complex device structures or high concentrations in solution.
3Measurement precision
If the second binding member is immobilized on the solid surface, then the binding capability is maintained, but the device structure becomes more complex
Solution Approach 1:
The solid surface serves multiple functions: it supports the first binding member, provides a platform for immobilizing the second binding member, and acts as the detection surface. This multi-functionality eliminates the need for separate chambers or complex structures, as the single solid surface performs all necessary roles in the assay.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for precise target detection without a separate chamber for the second binding member, simplifying device structure and improving assay accuracy and stability by maintaining the second binding member's binding capability over time.
Implementation Method 1
a first binding member which is immobilized on the first region of the solid surface, and a second binding member which is immobilized on the first region and the second region of the solid surface
Data Source
AI summary
Disclosed are an article for assaying a target, wherein the article includes a solid surface on which a first binding member, a blocking material, and a second binding member are immobilized, a method of manufacturing the article, and a method of detecting a target using the article.