Biological Cleanliness Measurement Kit Using ATP-ADP Conversion
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for measuring the cleanliness of bio-related samples and instruments, particularly those involving blood, are inadequate as they rely solely on ATP measurement, which is susceptible to degradation over time and temperature, leading to inaccurate detection of contamination.
Innovation Solution
The method involves measuring ATP, ADP, and AMP levels using enzymes that catalyze reactions to produce ATP from ADP and ADP from AMP, ensuring stable detection even after ATP degradation, using a kit comprising luciferin, luciferase, and metal salts with enzymes like pyruvate kinase and pyruvate orthophosphate dikinase.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If ATP measurement alone is used to evaluate cleanliness, then the measurement method is simple, but the measurement accuracy deteriorates due to ATP degradation over time and temperature
Solution Approach 1:
The invention segments the measurement approach by separately detecting ATP and ADP levels rather than measuring total nucleotides as a single value. This segmentation allows identification of degradation patterns (ATP converting to ADP over time) and provides more accurate cleanliness assessment by distinguishing between original ATP and degradation products
Solution Approach 2:
The invention uses ADP as an intermediary indicator to track ATP degradation. By measuring both ATP and ADP levels, the system can calculate the ratio and determine the extent of degradation, serving as a mediator between the original measurement target (ATP) and the actual cleanliness status
2Loss of time
If only ATP level is measured, then the measurement process is quick and simple, but the reliability deteriorates because ATP degrades to ADP and AMP over time
Solution Approach 1:
The invention performs preliminary measurement of both ATP and ADP levels immediately after sample collection, before significant degradation occurs. This preliminary action establishes a baseline that accounts for any ongoing degradation, allowing reliable cleanliness assessment even if measurement is delayed
Solution Approach 2:
The invention introduces feedback by calculating the ratio of ATP to (ATP+ADP) and using this ratio to assess cleanliness. The system continuously monitors this ratio to detect degradation trends and adjusts the interpretation of results based on the observed degradation level, providing reliable assessment despite time delays
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for accurate detection of biogenic contamination on bio-related samples and instruments, providing a more reliable assessment of cleanliness by maintaining luminescence levels and correlating with total ATP and AMP presence, even after heat or time-induced degradation.
Implementation Method 1
causing a reaction of ATP and the substrate, luciferin in the presence of luciferase and measuring luminescence
Implementation Method 2
Luciferin +ATP+O2→oxyluciferin+adenosine monophosphate (AMP)+pyrophosphoric acid (PPi)+CO2+light
Data Source
AI summary
Provided are a kit and a method for measuring cleanliness of a bio-related sample and a bio-related instrument, which is not susceptible to ATP-degrading activity. Provided are a method for measuring cleanliness of a bio-related sample or a bio-related instrument and a kit therefor, comprising using an enzyme that catalyzes a reaction that produces ATP from ADP, luciferin, luciferase, and a metal salt. Also provided are a method further comprising using pyruvate orthophosphate dikinase (PPDK), adenylate kinase (ADK), or pyruvate-water dikinase (PWDK) and a kit further comprising PPDK, ADK, or PWDK. Further provided are a method for measuring cleanliness of a bio-related sample or a bio-related instrument and a kit therefor, comprising using an enzyme that catalyzes a reaction that produces ATP from AMP, an enzyme that catalyzes a reaction that produces AMP from ADP, luciferin, luciferase, and a metal salt.