ATP Quantification Device Differential Luminescence Measurement
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Solution Overview
Problem
Conventional ATP quantification methods struggle to accurately measure minute ATP amounts in samples due to interference from processing reagents and environmental factors like optical transparency and color, leading to inaccurate results at the amol level.
Innovation Solution
A method and device that quantify ATP by calculating the differential luminescence between the ATP in the sample and the ATP extraction reagent, using a device with an ATP separation/collection mechanism and an ATP quantification mechanism to isolate and measure ATP, employing a hydrophilic and hydrophobic filter system and a liquid dispensing unit to separate and dispense reagents for precise luminescence measurement.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional ATP quantification method is used, then the measurement process is simple, but the measurement precision deteriorates due to interference from processing reagents and environmental factors
Solution Approach 1:
The measurement process is divided into separate stages: first measuring luminescence of ATP in the sample, then measuring luminescence of the processing reagent alone, and finally calculating the differential value. This segmentation allows each measurement to be optimized independently, improving overall precision while maintaining manageable complexity.
Solution Approach 2:
The invention extracts and measures the interfering component (ATP in processing reagent) separately from the sample measurement. By taking out the reagent's ATP contribution and measuring it independently, the interference is quantified and subtracted, significantly improving measurement precision.
2Reliability
If processing reagent is added to remove quantification inhibitors, then the reliability of measurement is improved, but the measurement precision deteriorates due to ATP contained in the reagent itself
Solution Approach 1:
The invention converts the harmful effect (ATP in reagent causing positive interference) into a beneficial measurement approach. By measuring the reagent's ATP luminescence separately and subtracting it from the total measurement, the previously harmful interference becomes a correctable factor, improving both reliability and precision.
Solution Approach 2:
The invention introduces a differential measurement approach as an intermediary calculation step. Instead of directly measuring sample ATP, it uses the difference between two measurements (sample with reagent and reagent alone) to obtain the accurate ATP amount, eliminating reagent interference.
3Measurement precision
If minute ATP amounts are measured directly, then the sensitivity requirement is high, but the measurement precision deteriorates due to background interference
Solution Approach 1:
The invention extracts the background interference (reagent ATP luminescence) as a separate measurable quantity. By measuring and subtracting this background component, the precision of minute ATP detection is significantly improved, allowing accurate measurement even at amol levels.
Solution Approach 2:
The invention implements a feedback mechanism where the reagent's ATP luminescence measurement feeds into the final calculation. This feedback allows the system to automatically compensate for background interference, improving precision without requiring manual correction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for highly sensitive and accurate quantification of ATP in samples, minimizing interference from processing reagents and environmental factors, thereby improving the precision of ATP measurement at the amol level.
Implementation Method 1
causing an ATP extraction reagent to contact microbes contained in a sample; extracting endogenous ATP from the microbes
Implementation Method 2
counting the number of the microbes in accordance with an amount of luminescence when the ATP is reacted with a luminescent reagent
Data Source
Figure 1
Figure 2
Figure 3A~3B
AI summary
The present invention is a device (1) for quantifying biological material (ATP) of cells contained in a liquid sample (8a) as a sample. The device (1) includes a controller (34) that calculates an amount of the biological material of cells contained in the liquid sample (8a), based on a differential amount of luminescence between an amount of luminescence when an ATP luminescence reagent (8b) as a luminescent reagent for biological material is reacted with the the biological material (ATP) that is separated and extracted from cells contained in the liquid sample (8a) by bringing an ATP extraction reagent (8c) as a processing reagent into contact with the liquid sample (8a), and an amount of luminescence when the ATP luminescent reagent (8b) is reacted with the ATP extraction reagent (8c).