AtSRP Gene Overexpression for Abiotic Stress Tolerance
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Solution Overview
Problem
Current methods are inadequate for enhancing crop plant tolerance to abiotic stresses such as drought, high salt, and cold, limiting their growth and productivity.
Innovation Solution
Introduction of nucleotide sequences encoding Arabidopsis thaliana stress-related protein (AtSRP) genes, which are homologs to the small rubber particle protein in rubber trees, to increase plant tolerance to abiotic stresses by over-expressing these genes in plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional breeding and traditional stress tolerance methods are used, then crop plants can maintain basic growth, but they cannot achieve sufficient tolerance to severe abiotic stresses such as drought, high salt, and cold
Solution Approach 1:
The patent changes the genetic parameters of plants by introducing and over-expressing AtSRP genes from Arabidopsis thaliana. This genetic parameter change enables plants to achieve both high stress tolerance and maintained growth rates, resolving the contradiction between reliability under stress and productivity.
Solution Approach 2:
The patent creates composite genetic structures by combining AtSRP genes with plant genomic DNA. This composite approach integrates stress tolerance functions into the plant genome, enabling simultaneous achievement of stress resistance and normal growth functions that were previously contradictory.
2Reliability
If stress tolerance is enhanced through traditional methods, then plants can survive adverse conditions, but their growth rate and productivity are significantly reduced
Solution Approach 1:
By over-expressing AtSRP genes, the patent changes the physiological parameters of plants to achieve high survival rates under stress while maintaining growth rates. The genetic over-expression enables plants to allocate resources efficiently between stress defense and growth, resolving the speed-reliability contradiction.
3Reliability
If plants are exposed to abiotic stresses to select for tolerant varieties, then stress tolerance can be improved, but water availability and growing conditions are severely limited
Solution Approach 1:
The patent applies preliminary action by genetically modifying plants with AtSRP genes before exposing them to stress conditions. This pre-equipping of stress tolerance mechanisms eliminates the need to severely limit water availability for selection, as tolerant plants can be created through genetic engineering rather than harsh environmental selection.
Data Source
AI summary
The present invention provides a method for improving the tolerance of a plant to an abiotic stress and a method for promoting growing of a plant, comprising a nucleotide sequence encoding the AtSRP (Arabidopsis thaliana stress related protein) of a plant. The present nucleotide sequence is involved in abiotic stress tolerance such as drought, low-temperature and salt stresses of plants. Therefore, the overexpressing transgenic plants have excellent tolerances to these abiotic stresses, whereby they may be useful as novel functional crops which are affected by climates and environments of the cultivated areas. In addition, where the plants are transformed with the present nucleotide sequence, the growth abilities of the transgenic plants are remarkably enhanced, whereby they may effectively used for cultivating the plants with novel function of rapid growing, and biomass.