Attachment Solution for Cytological Sample Adhesion
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Solution Overview
Problem
Current diagnostic methods face challenges in maintaining cytological and histological samples adhered to substrates during analysis, which is crucial for obtaining valuable diagnostic information.
Innovation Solution
An attachment solution comprising an attachment base, such as alcohol or ketones, combined with a nonsteroidal anti-inflammatory drug and an anti-coagulant, is used to adhere samples to substrates, preventing cross-linking of proteins and allowing for efficient fixation and imaging without additional processing steps like antigen retrieval.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional diagnostic methods are used to analyze histological and cytological samples, then sample processing can proceed through standard protocols, but the samples may not remain adhered to the substrate, resulting in loss of valuable diagnostic information
Solution Approach 1:
The patent applies preliminary action by treating the substrate with a specific coating composition before sample application. This pre-treatment creates adhesive properties on the substrate surface that ensure sample adherence during subsequent processing steps, preventing sample loss and preserving diagnostic information throughout the diagnostic workflow
Solution Approach 2:
The patent uses a coating composition as an intermediary substance between the substrate and the biological sample. This coating layer acts as a mediator that promotes adhesion between the sample and substrate while maintaining compatibility with subsequent diagnostic processing steps, solving the adherence problem without interfering with diagnostic analysis
2Reliability
If additional processing steps like antigen retrieval are performed to ensure sample adherence, then sample retention on substrate is improved, but the complexity and time of the diagnostic process increases
Solution Approach 1:
The patent eliminates the need for subsequent antigen retrieval steps by performing the adhesion-promoting action in advance through substrate coating. This preliminary treatment ensures sample retention throughout processing without requiring additional complex steps later in the workflow, reducing overall process complexity while maintaining reliable sample adherence
3Ease of manufacture
If standard substrate coating methods are used, then substrate preparation is simple, but the samples do not adhere reliably during subsequent processing
Solution Approach 1:
The patent uses a composite coating composition containing multiple components (such as poly-L-lysine, gelatin, and other adhesion-promoting substances) applied to the substrate. This composite material provides reliable sample adherence while maintaining compatibility with standard substrate preparation methods, achieving both ease of manufacture and reliable sample retention
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively adheres samples to substrates, enabling reliable diagnostic analysis by preventing sample cracking and coagulation, allowing for rapid curing and compatibility with various analysis techniques.
Implementation Method 1
The attachment solution includes an attachment base and a nonsteroidal anti-inflammatory drug... The sample is dispensed onto an analysis platform as one or more droplets and cured. The sample may then be fixed...
Implementation Method 2
An attachment solution comprising an attachment base, such as alcohol or ketones, combined with a nonsteroidal anti-inflammatory drug and an anti-coagulant, is used to adhere samples to substrates, preventing cross-linking of proteins
Implementation Method 3
An attachment solution comprising an attachment base, such as alcohol or ketones, combined with a nonsteroidal anti-inflammatory drug and an anti-coagulant, is used to adhere samples to substrates
Implementation Method 4
The sample is dispensed onto an analysis platform as one or more droplets and cured
Data Source
AI summary
This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.