Attenuated Pasteurella multocida Strains for Vaccine Efficacy
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current vaccines against Pasteurella multocida do not adequately protect against diseases caused by this bacterium, particularly in livestock and companion animals, as they fail to induce robust and long-lasting immunity.
Innovation Solution
Development of attenuated Pasteurella multocida strains with specific genetic deletions, such as in the hyaD gene for acapsular phenotype and nanP/nanU genes for sialic acid residue addition, which are used to create vaccine compositions that can be administered orally or through other routes to induce protective immunity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current vaccines against Pasteurella multocida are used, then vaccination can be administered, but they fail to induce robust and long-lasting immunity
Solution Approach 1:
The patent applies parameter changes by modifying specific genetic parameters of the Pasteurella multocida bacterium. Deletions are introduced in the hyaD gene (affecting capsule synthesis) and nanP/nanU genes (affecting sialic acid addition), thereby changing the bacterial phenotype to create an attenuated strain that serves as a more effective vaccine while inducing robust and long-lasting immunity
2Reliability
If wild-type P. multocida is used for vaccination, then immune response can be induced, but safety concerns arise due to virulence
Solution Approach 1:
The patent applies the taking out principle by removing specific virulence-related genetic elements from the Pasteurella multocida bacterium. The hyaD gene deletion eliminates capsule synthesis capability, and the nanP/nanU gene deletion removes sialic acid addition ability, thereby extracting the harmful virulence factors while preserving the bacterium's ability to induce protective immunity
Solution Approach 2:
The patent converts the harmful virulence factors into beneficial vaccine characteristics. By deleting the hyaD and nanP/nanU genes, the bacterium loses its pathogenic capabilities (capsule and sialic acid-mediated virulence) but gains enhanced vaccine efficacy through attenuated phenotype, transforming a harmful pathogen into a safe and effective vaccine strain
Data Source
AI summary
This disclosure provides attenuated P. multocida strains which can be used to prepare vaccine compositions useful for protection against P. multocida.