Autofluorescence Cell Sorting for Photosynthetic Dye Production
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Solution Overview
Problem
Current methods for obtaining photosynthetic dye-containing cells with suppressed photosynthetic-dye decrease are complex and time-consuming, requiring multiple culture steps and large numbers of culture plates.
Innovation Solution
A method involving the measurement of autofluorescence spectra of cultured photosynthetic dye-containing cells to identify and sort cell populations with suppressed photosynthetic-dye decrease, using a cell analyzer and/or cell sorter.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional culture methods are used to obtain photosynthetic dye-containing cells with suppressed photosynthetic-dye decrease, then the desired cell population can be obtained, but the process becomes complicated and time-consuming requiring multiple culture steps and large numbers of culture plates
Solution Approach 1:
The patent replaces the conventional mechanical culture method (multiple culture plates and repeated culture steps) with a fluorescence-activated cell sorting (FACS) system. By measuring autofluorescence spectra of photosynthetic dye-containing cells and sorting based on fluorescence intensity, the desired cell population is obtained in a single step without repeated culture operations, thereby resolving the contradiction between reliability of obtaining desired cells and time consumption.
2Reliability
If conventional culture methods are used to obtain photosynthetic dye-containing cells with suppressed photosynthetic-dye decrease, then the desired cell population can be obtained, but the process becomes complicated requiring large numbers of culture plates
Solution Approach 1:
The patent replaces the complex mechanical culture system (multiple culture plates, repeated culture steps) with an automated fluorescence-activated cell sorting system. The FACS apparatus measures autofluorescence spectra and sorts cells based on fluorescence intensity characteristics, eliminating the need for multiple culture plates and complex culture protocols while maintaining the ability to obtain the desired cell population.
Solution Approach 2:
The patent employs autofluorescence measurement of the photosynthetic dye-containing cells themselves for identification and sorting. The cells' intrinsic fluorescence properties serve as the sorting criterion without requiring external labels or complex preparation, enabling the system to automatically distinguish and sort desired cells based on their own characteristics.
3Reliability
If conventional culture methods are used to obtain photosynthetic dye-containing cells with suppressed photosynthetic-dye decrease, then the desired cell population can be obtained, but the process requires repeated culture operations
Solution Approach 1:
The patent replaces repeated culture operations with a single fluorescence-activated cell sorting operation. By measuring autofluorescence spectra and sorting based on fluorescence intensity, the desired cell population is obtained in one step rather than through multiple repeated culture steps, thereby significantly improving productivity and cell production efficiency.
Solution Approach 2:
The patent performs preliminary measurement of autofluorescence spectra to identify cells with suppressed photosynthetic-dye decrease before sorting. This preliminary characterization allows the system to pre-identify the desired cell population characteristics and sort them in a single operation, eliminating the need for repeated culture operations to enrich the desired population.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the efficient and selective isolation of cells with suppressed photosynthetic-dye decrease, enhancing the production of useful chemical substances like biofuels.
Implementation Method 1
a measurement step of measuring autofluorescence spectra of the cultured photosynthetic dye-containing cell group
Implementation Method 2
a sorting step of sorting a cell population having a suppressed photosynthetic-dye decrease from the photosynthetic dye-containing cell group on the basis of the measured autofluorescence spectra
Data Source
AI summary
An object of the present disclosure is to provide a technique for more efficiently obtaining a photosynthetic dye-containing cell having a desired characteristic.The present disclosure is directed to providing a method for sorting a photosynthetic dye-containing cell, including a measurement step of measuring autofluorescence spectra of the cultured photosynthetic dye-containing cell group; and a sorting step of sorting a cell population having a suppressed photosynthetic-dye decrease from the photosynthetic dye-containing cell group on the basis of the measured autofluorescence spectra. The present disclosure is also directed to providing a cell sorter and a cell sorting system configured to perform the sorting method.