Autofluorescent Substrate for Cell Shape Detection
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Solution Overview
Problem
Conventional methods for detecting cell infection rates require both phase contrast and fluorescent observation optical systems, making them cumbersome and inefficient for detecting the outer shape of cells in fluorescence detection systems.
Innovation Solution
A sample detection plate with a first substrate that emits autofluorescence in response to specific electromagnetic waves, allowing detection of the sample's outer shape using a fluorescent optical system without the need for a phase contrast observation system, comprising a first substrate with a sample container that absorbs electromagnetic waves and emits autofluorescence, which is then detected by a fluorescence detection device.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a phase contrast observation optical system is used to detect the outer shape of cells, then the outer shape detection is achieved, but the device complexity increases due to requiring multiple optical systems
Solution Approach 1:
The patent combines the outer shape detection function and fluorescent label detection function into a single fluorescent observation optical system. The substrate emits autofluorescence to define cell boundaries, while fluorescent labels indicate infected cells, both detected by the same optical system, eliminating the need for separate phase contrast and fluorescent observation systems.
Solution Approach 2:
The substrate acts as an intermediary that emits autofluorescence to define the outer shape of cells. This intermediary mechanism allows the fluorescent optical system to detect cell boundaries without requiring phase contrast optics, as the substrate's autofluorescence serves as a reference for identifying the outer shape of transparent cells.
2Measurement precision
If both phase contrast and fluorescent observation optical systems are used, then both outer shape detection and fluorescent label detection are achieved, but the detection efficiency decreases due to multiple systems
Solution Approach 1:
The fluorescent observation optical system is designed to perform multiple functions: detecting the outer shape of cells through substrate autofluorescence and detecting fluorescent labels on infected cells. This multi-functional approach eliminates the need for separate phase contrast observation, improving detection efficiency while maintaining accuracy for both cell boundary identification and infection rate calculation.
3Device complexity
If a fluorescent optical system alone is used without phase contrast, then the device complexity is reduced, but the outer shape detection capability is lost
Solution Approach 1:
The substrate serves as an intermediary that emits autofluorescence when excited by the fluorescent optical system. This autofluorescence provides a visual reference for the outer shape of transparent cells, enabling the fluorescent optical system alone to detect cell boundaries without requiring phase contrast optics. The substrate's fluorescent properties bridge the gap between simple optical system design and effective outer shape detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the detection of the outer shape of samples, such as cells, using a single fluorescent optical system, improving efficiency and simplifying the detection process by eliminating the need for phase contrast observation, and allowing for sharper image outlines and accurate counting of samples.
Implementation Method 1
The first substrate includes a first material that emits autofluorescence in response to the electromagnetic waves having the predetermined wavelength
Data Source
AI summary
A sample detection plate according to the present disclosure includes a first substrate having a first surface, and a sample container that is provided on the first surface of the first substrate to contain a sample that absorbs electromagnetic waves having a predetermined wavelength. The first substrate includes a first material that emits autofluorescence in response to the electromagnetic waves having the predetermined wavelength.