Automated Multi-Module Cell Editing Instruments
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Solution Overview
Problem
Current genome editing methods with engineered nucleases are not compatible with automation due to low efficiencies and challenges with cell transformation, growth measurement, and selection, limiting the complexity and nature of cell populations that can be created.
Innovation Solution
Automated multi-module cell editing instruments and systems that integrate nucleic acid introduction, editing, and cell growth, allowing for recursive genome editing and the generation of libraries of living cells with desired genomic changes using nuclease-directed techniques.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Extent of automation
If traditional benchtop devices are used for genome editing, then manual operation is possible, but automation compatibility is poor and scaling is limited
Solution Approach 1:
The system is divided into discrete modular components including a chassis, replaceable cartridges (reagent cartridge, wash cartridge), and distinct modules (electroporation module, growth module, filtration module). Each module performs a specific function and can be independently replaced or configured, enabling automation while managing complexity through standardized interfaces and protocols.
2Productivity
If genome editing is performed manually, then flexibility in protocol adjustment is maintained, but editing efficiency is low and throughput is limited
Solution Approach 1:
The system performs preliminary actions by pre-configuring cartridges with reagents, pre-programming automated protocols for electroporation parameters and timing, and pre-establishing growth conditions in the growth module. This automation of preparatory steps increases editing efficiency while reducing the operational burden on users during the actual editing process.
3Adaptability or versatility
If simple cell processing is used, then current methods can be applied, but the complexity and nature of cell populations that can be created is limited
Solution Approach 1:
The instrument is designed with universal multi-functional modules that can handle diverse cell types and editing applications. The electroporation module can process different cell suspensions, the growth module can support various cell growth conditions, and the filtration module can separate cells based on different criteria. This universality enables creation of diverse cell populations without proportionally increasing instrument complexity.
Data Source
AI summary
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.