Automated Cell Isolation for Aseptic Tissue Processing
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Solution Overview
Problem
Cell isolation procedures are time-consuming and prone to human error, requiring manual operations that are costly and difficult to standardize, especially for different types of tissues, and there is a need for a device that minimizes human intervention and ensures aseptic conditions for safe and reliable cell isolation.
Innovation Solution
An automatically operable cell isolation device with a liquid distribution unit, biopsy kit, disposable unit, and valve array, along with electrical components and a method for performing cell isolation procedures, minimizing human intervention and ensuring aseptic conditions, capable of isolating cells from various tissues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If manual cell isolation procedures are used, then flexibility in handling different tissue types is maintained, but human error increases and productivity decreases
Solution Approach 1:
The automated cell isolation device performs operations autonomously without continuous human intervention. The system self-regulates fluid flow, tissue processing, and cell collection through programmed sequences, eliminating manual operations while maintaining reliability across different tissue types.
Solution Approach 2:
The device accommodates different tissue types by programmatically adjusting processing parameters such as enzyme concentration, incubation time, and mechanical agitation intensity. This allows the same automated system to reliably handle varied tissues without manual reconfiguration.
2Productivity
If automated cell isolation device is implemented, then productivity increases and human error decreases, but device complexity increases
Solution Approach 1:
The automated cell isolation device is designed as a multi-functional system that can process various tissue types using the same core apparatus. The valve array and fluid distribution unit can be reprogrammed to handle different isolation protocols, reducing the need for multiple specialized devices.
Solution Approach 2:
The device incorporates a modular valve array with multiple independently controllable valves that can be programmed to execute different isolation sequences. This segmentation allows complex multi-step procedures to be broken down into manageable, automated operations.
3Adaptability or versatility
If manual operations are performed, then adaptability to different tissue types is maintained, but loss of time increases
Solution Approach 1:
The system pre-programs multiple isolation protocols for different tissue types, allowing rapid selection and execution without manual setup. Common operations are prepared in advance and can be automatically initiated, significantly reducing preparation time while maintaining adaptability.
Solution Approach 2:
The automated device performs cell isolation operations continuously without interruption by manual handling. Fluid flow, enzyme treatment, and cell collection proceed in seamless sequences, eliminating downtime between operations and reducing total processing time.
4Object-affected harmful factors
If aseptically closed device is used, then contamination risk is reduced, but ease of manufacture decreases
Solution Approach 1:
The device employs disposable components such as single-use fluid containers and disposable valve arrays that are pre-sterilized. These components are discarded after one use, eliminating the need for complex sterilization systems while ensuring aseptic conditions and simplifying manufacturing requirements.
Data Source
AI summary
The present invention relates to a device and method for automatically isolating viable cells from connective, epithelial or other tissue, and, if the tissue is multilayered, for automatically separating one tissue layer from another.