Automated Nucleic Acid Extraction With Disposable Suction Tubes
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Solution Overview
Problem
Existing nucleic acid extraction methods face challenges such as complexity, high cost, long processing times, instability, and risk of cross-contamination, particularly in automated systems.
Innovation Solution
A nucleic acid extraction device featuring a sliding seat with sample and reagent strip supports, a liquid transfer table with piston assemblies and suction assemblies, and disposable capillary suction tubes to facilitate automated, high-purity extraction without cross-contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual nucleic acid extraction methods are used, then flexibility and adaptability are maintained, but productivity is low and labor intensity is high
Solution Approach 1:
The extraction system is divided into separate functional modules: a lysis module for sample preparation, an extraction module for nucleic acid separation, and a purification module for final cleaning. Each module performs a specific function independently, enabling automated high-throughput processing while maintaining operational flexibility through modular configuration.
Solution Approach 2:
The apparatus is designed with universal components that can handle multiple sample types and extraction protocols. The same basic system can process DNA, RNA, and other nucleic acids through programmable control, allowing one device to replace multiple specialized manual procedures.
2Productivity
If automated extraction systems are implemented, then productivity increases, but ease of operation decreases due to complexity
Solution Approach 1:
The system incorporates self-service features including automatic sample tracking, real-time progress monitoring, and self-diagnostic functions. The microprocessor-controlled system automatically manages the extraction process parameters, reducing the need for user intervention while maintaining high productivity.
Solution Approach 2:
The apparatus includes feedback mechanisms where sensors monitor extraction progress and system status in real-time. The microprocessor adjusts operational parameters based on feedback from various sensors, ensuring optimal performance while simplifying operation through automated control loops.
3Measurement precision
If conventional extraction methods are used, then device complexity is low, but measurement precision and extraction quality are insufficient
Solution Approach 1:
The system replaces manual mechanical operations with automated mechanical systems controlled by microprocessors. Precision instrumentation including automated pipetting systems, controlled temperature incubators, and electronic sensors substitute for manual techniques, achieving consistent high-quality extraction results.
Solution Approach 2:
The apparatus precisely controls critical extraction parameters including temperature, pH, incubation time, and reagent volumes through electronic control systems. This precise parameter management ensures reproducible high-quality nucleic acid extraction while maintaining system complexity at acceptable levels through automation.
4Loss of time
If manual extraction procedures are performed, then adaptability to different samples is maintained, but loss of time increases
Solution Approach 1:
The automated system enables continuous extraction processing with minimal idle time. Samples can be processed in parallel across multiple channels, and the system continuously monitors and adjusts parameters to maintain optimal extraction conditions throughout the cycle, significantly reducing total processing time compared to sequential manual operations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, automated nucleic acid extraction with high purity and reduced risk of cross-contamination, achieving efficient and cost-effective results in a short time frame.
Implementation Method 1
a magnetic bead having a nucleic acid binding capability and a magnetic property
Data Source
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AI summary
A nucleic acid extraction apparatus comprises a workbench having a table top, and further comprises a sliding seat, a sample rack, a reagent strip rack, a pipetting table, a piston assembly and a suction assembly. The nucleic acid extraction apparatus comprises the sliding seat and the pipetting table, the sample rack and the reagent strip rack are arranged on the sliding seat, the piston assembly and the suction assembly are respectively arranged on the pipetting table, the sample rack is provided with sample holes, the reagent strip rack is provided with accommodation slots, the number of accommodation slots corresponds to that of the sample holes on a one-to-one basis, and when reagent strips are respectively accommodated in the accommodation slots, a first suction head hole, second suction head holes and various reagent holes in each reagent strip respectively correspond to the corresponding sample holes, such that the loading of a suction head assembly and suction heads and operations such as suction, injection and mixing of a sample and various reagents can be realized by means of a vertical movement, relative to the sliding seat, of the pipetting table and a horizontal movement, relative to the pipetting table, of the sliding seat, thereby realizing the automated operation of nucleic acid extraction.