Automated Perifusion Device for Parallel Cell Stimulus Analysis
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Solution Overview
Problem
Current perifusion methods for testing cell viability are time and labor intensive, requiring manual sampling and analysis of multiple samples from multiple columns, which limits data collection efficiency.
Innovation Solution
A perifusion device with a sample container and receptacle housing system that allows for automated collection and analysis of samples from multiple columns, using a programmable drive and valve manifold to control fluid flow and stimulus delivery, enabling efficient data collection and cell response detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual sampling and analysis methods are used for perifusion experiments, then the process can be performed with simple equipment, but the process becomes time and labor intensive with reduced productivity
Solution Approach 1:
The system divides the sampling task into multiple automated receptacles that can simultaneously collect samples from multiple columns, segmenting the manual operation into parallel automated processes
Solution Approach 2:
The automated drive system and receptacle housing enable the system to perform sampling and collection operations autonomously without requiring manual intervention for each sample
2Quantity of substance
If multiple samples are run simultaneously from multiple columns, then sufficient data can be provided, but the complexity of operating and managing multiple columns increases
Solution Approach 1:
The receptacle housing with multiple receptacles serves multiple functions: it can simultaneously receive samples from multiple columns, store samples, and be systematically moved by the automated drive, consolidating multiple operations into a single universal component
Solution Approach 2:
The programmable control system coordinates the drive movement and sample collection timing, providing systematic feedback control to manage the complexity of simultaneous multi-column operations
3Extent of automation
If automated collection systems are implemented, then labor requirements are reduced and productivity increases, but the device complexity increases
Solution Approach 1:
The system merges the drive mechanism, receptacle housing, and sample collection functionality into an integrated automated assembly, combining multiple components into a unified system that reduces overall complexity
Data Source
AI summary
A perifusion device includes at least one sample container for cells, the sample container having an inlet and an outlet. The container receives test liquid through the inlet and discharges the liquid through the outlet. A manifold having a plurality of liquid inlets, control valves, and liquid outlets can be provided. A receptacle housing has a plurality of receptacles. A drive is connected to the receptacle housing for moving the receptacle housing. A programmable controller can be provided to control movement of the receptacle housing. The test liquid includes at least one stimuli for the cells. The liquid collected in the receptacles is analyzed to determine the response of the cells to the stimuli.