Automatic Extraction Device for Biological Sample Purification
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Solution Overview
Problem
Current bioengineering methods for extracting or purifying target biological substances, such as plasmids, rely heavily on manual operations, leading to inefficiencies in throughput, accuracy, and consistency, particularly when handling large numbers of samples.
Innovation Solution
An automatic extraction device comprising a sample processing apparatus with uniform mixing and magnetic attraction mechanisms, a consumable assembly, and a pipetting apparatus, which automates the extraction process by adding reagents, mixing, and separating target substances using magnetic beads.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual operations are used for extraction and purification, then flexibility in process selection is maintained, but operator workload increases and throughput decreases
Solution Approach 1:
The automated extraction device performs extraction and purification operations autonomously without requiring continuous manual intervention. The system self-manages the entire workflow including reagent addition, mixing, magnetic separation, and sample processing, thereby reducing operator workload while maintaining high throughput capability
Solution Approach 2:
Manual mechanical operations are replaced by an automated mechanical system comprising robotic arms, automated pipettes, and magnetic separation mechanisms. This substitution enables consistent high-speed operation while eliminating the limitations of manual dexterity and endurance
2Manufacturing precision
If manual operations are used, then process adaptability is maintained, but individual differences affect accuracy and consistency
Solution Approach 1:
The system replaces manual operations with automated mechanical and electronic control systems that execute extraction protocols with precise repeatability. Automated pipetting, mixing, and magnetic separation eliminate variability introduced by different operators, ensuring consistent accuracy across all samples
Solution Approach 2:
The automated system incorporates sensors and control algorithms that monitor and adjust operational parameters in real-time, ensuring consistent execution of extraction protocols. This feedback mechanism maintains high precision while managing the complexity of automated operations
3Productivity
If manual processing is used, then device simplicity is maintained, but detection throughput is limited
Solution Approach 1:
The system autonomously manages high-throughput processing workflows, automatically loading samples, reagents, and consumables, then executing extraction and purification sequences without operator intervention. This self-service capability enables sustained high throughput while managing automation complexity internally
Solution Approach 2:
The automated system divides the extraction process into discrete modular steps (reagent addition, mixing, magnetic separation, supernatant removal, bead resuspension) that can be executed in parallel across multiple samples, thereby increasing throughput while keeping each individual operation simple and manageable
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device significantly reduces operator workload, enhances accuracy and consistency, and enables high-throughput processing of biological samples by automating the extraction and purification of target substances like plasmids, proteins, and nucleic acids.
Implementation Method 1
a magnetic attraction mechanism, which are respectively configured to perform uniform mixing processing and magnetic attraction processing on a biological sample
Implementation Method 2
The uniform mixing mechanism is configured to perform uniform mixing processing on a biological sample
Data Source
AI summary
An automatic extraction device and a use method therefor. The automatic extraction device comprises a sample processing apparatus (100), a portal frame (200), a consumable assembly (300), and a pipetting apparatus (400). The sample processing apparatus (100) comprises a uniform mixing mechanism (130) and a magnetic attraction mechanism (140), which are respectively used for performing uniform mixing processing and magnetic attraction processing on a biological sample. The portal frame (200) comprises a base (201) and a moving beam (202) capable of moving relative to the base (201). The sample processing apparatus (100) and the consumable assembly (300) are provided on the base (201). The pipetting apparatus (400) is provided on the moving beam (202) for implementing a pipetting operation between the consumable assembly (300) and the sample processing apparatus (100). The automatic extraction device can be used for extracting and purifying a target biological substance, such that mechanical automation of an extraction or purification process can be achieved, thereby reducing the time and energy of operators while improving the extraction and purification efficiency.