Automatic Slicing and Collecting Device for Vibrating Microtome Sections

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Solution Overview

Problem

Manual collection of thin sections from vibrating microtomes in biological research and medical examination is inefficient and laborious, and existing automatic devices are complex and expensive due to the need for multiple water channels and controllers.

Innovation Solution

An automatic slicing and collecting device comprising a tank filled with a buffer solution, a vibrating microtome, pipelines with valves and a pump, and a dispenser head with an annular sieve, utilizing a reversible motor and three-axis manipulator to automate the collection of sliced sections onto a perforated plate, allowing for efficient and continuous section collection.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If manual collection of thin sections is used, then operation simplicity is maintained, but productivity is low and labor is intensive

Engineering Contradiction:
Improvesection collection efficiencyVSAvoidmanual operation requirement
Core Design Contradiction:
ProductivityVSEase of operation

Solution Approach 1:

The system performs automatic section collection through self-service mechanisms. The pump automatically draws buffer solution through the microtome and dispensing needle, the valve controls automatic section transfer to the well plate, and the manipulator autonomously positions components. This eliminates manual section picking while maintaining operational simplicity through automated self-regulating processes.

Inventive Principle:
Principle #25Self-service

2Productivity

If multiple water channels and controllers are used for automatic collection, then productivity is improved, but device complexity increases and manufacturing cost rises

Engineering Contradiction:
Improveautomatic section collection efficiencyVSAvoidnumber of water channels and controllers
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent merges multiple functions into a single integrated system. The pump, valve, and manipulator work as a unified automated assembly rather than separate complex water channel systems. The buffer solution serves dual purposes: as a medium for section transport and as a collection vehicle, eliminating the need for separate water channels and controllers while achieving automatic high-throughput section collection.

Inventive Principle:
Principle #5Merging (Combining)

3Productivity

If a pump and valve system is used for section collection, then productivity is improved, but device complexity increases

Engineering Contradiction:
Improveautomated section transfer speedVSAvoidpipeline and control component quantity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The buffer solution performs multiple functions simultaneously: it acts as a transport medium for sections, a collection vehicle for automated dispensing, and a rinsing agent. The single pump-valve-pipeline system handles both section transfer and solution circulation, eliminating the need for separate water channel systems and reducing overall device complexity while maintaining high productivity.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The device significantly improves the efficiency of slicing and collecting by automating the process, reducing manual labor and complexity, while being easier to assemble and operate, with the ability to collect sections automatically and return buffer solution to the tank.

Implementation Method 1

The pump comprises a reversible motor. One end of the first pipeline is connected to the vibrating microtome to collect sections, and the other end of the first pipeline is connected to a pump. The first pipeline is provided with a first valve, and a filter is disposed between the pump and the first pipeline.

Methodology Applied
Scientific EffectReversible motor rotation:

Implementation Method 2

One end of the first pipeline is connected to the vibrating microtome to collect sections, and the other end of the first pipeline is connected to a pump

Methodology Applied
Scientific EffectPumping: Pump

Implementation Method 3

a filter is disposed between the pump and the first pipeline

Methodology Applied
Scientific EffectFiltration: Filter (physical)

Implementation Method 4

an annular sieve is sleeved on the dispenser head, and a diameter of the annular sieve equals to a diameter of pores on the perforated plate

Methodology Applied
Scientific EffectSieve filtration: Filter (physical)

Data Source

PatentUS10197476B2Automatic slicing and collecting device and method for slicing and collecting using the device
Publication Date: 2019.02.05 HUAZHONG UNIV OF SCI & TECH
  • US10197476B2 patent drawing
  • US10197476B2 patent drawing
  • US10197476B2 patent drawing

AI summary

An automatic slicing and collecting device, the device including: a first tank, a vibrating microtome, a first pipeline, and a second pipeline. The first tank is filled with a buffer solution. The vibrating microtome is disposed in the first tank. One end of the first pipeline is connected to the vibrating microtome to collect sections, and the other end of the first pipeline is connected to a pump. The pump includes a reversible motor. The first pipeline is provided with a first valve, and a filter is disposed between the pump and the first pipeline. One end of the second pipeline is disposed between the first valve of the first pipeline and the pump, and the second pipeline is provided with a second valve.