Chromatography Autosampler Cell Identification via Machine Vision

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Solution Overview

Problem

Current autosamplers require manual labeling and placement of vials, leading to high error rates due to the complexity of managing multiple vials in a sample tray, with little to no chain-of-custody assurance once vials are loaded.

Innovation Solution

A method and system utilizing a machine vision module within the autosampler to scan the end surfaces and side walls of sample cells, combined with a cell gripper assembly to grip, lift, and rotate the cells, allowing for automated identification and positioning of sample cells within the autosampler.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If manual labeling and placement of vials is used, then users can identify samples, but error rates increase due to complexity of managing multiple vials

Engineering Contradiction:
Improvesample identification accuracyVSAvoidmanual tracking complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent replaces the manual mechanical process of labeling and tracking vials with an automated machine vision system using cameras and image processing algorithms. The system automatically captures images of vial labels, processes them through OCR or pattern recognition, and matches them to the correct positions in the sample tray, eliminating manual intervention and reducing errors.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The system enables self-service by allowing the autosampler to automatically identify and locate samples without user intervention. The machine vision system independently performs label recognition, position determination, and verification, making the system self-sufficient in sample identification tasks.

Inventive Principle:
Principle #25Self-service

2Reliability

If manual placement of vials in sample tray is used, then users can load samples, but chain-of-custody assurance is lost once vials are placed

Engineering Contradiction:
Improvechain-of-custody trackingVSAvoidautomated identification
Core Design Contradiction:
ReliabilityVSExtent of automation

Solution Approach 1:

The patent implements feedback by continuously monitoring and verifying sample positions through machine vision. The system captures images of the sample tray, automatically identifies vial positions and labels, and provides real-time verification to the control system. This closed-loop feedback ensures chain-of-custody tracking is maintained throughout the autosampler operation.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The machine vision system acts as an intermediary between the physical sample tray and the digital control system. It bridges the gap by capturing visual information from the physical world, processing it through image recognition, and translating it into digital data that the control system can use for tracking and verification.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Measurement precision

If users manually track vial positions, then sample locations can be recorded, but significant user error occurs in placement

Engineering Contradiction:
Improvevial position accuracyVSAvoidmanual tracking difficulty
Core Design Contradiction:
Measurement precisionVSEase of operation

Solution Approach 1:

The patent replaces manual visual tracking and recording with an automated machine vision system. Cameras capture high-resolution images of the sample tray, and image processing algorithms automatically determine the precise position of each vial. This eliminates the need for users to manually track and record positions, thereby eliminating human error in position measurement.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Productivity

If sample trays have capacities of 50 or more vials, then throughput increases, but error probability increases due to complexity

Engineering Contradiction:
Improvesample tray capacityVSAvoidcorrect placement assurance
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The system enables self-service by allowing the autosampler to automatically identify, verify, and track each vial independently. The machine vision system autonomously processes all vials in the tray, recognizing labels and determining positions without user intervention, which is essential for maintaining reliability when handling large numbers of samples.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent implements comprehensive feedback by monitoring all vial positions and labels simultaneously through the machine vision system. The system provides real-time verification for each vial placement, comparing actual positions against the expected configuration, and can alert or correct errors automatically, ensuring high reliability even with 50 or more vials in the tray.

Inventive Principle:
Principle #23Feedback

Data Source

PatentEP4560322A1Identification of sample cells in a chromatography autosampler
Publication Date: 2025.05.28 DIONEX CORP
  • EP4560322A1 patent drawingFigure 1
  • EP4560322A1 patent drawingFigure 2A~2F
  • EP4560322A1 patent drawingFigure 3A~4

AI summary

Methods and systems are provided for the identification of sample cells in a sample tray that is placed in a chromatography autosampler. A cell gripper and labels are also provided to facilitate such identification.