B Lymphocyte Administration for Tissue Repair and Cardiac Function
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current treatments for tissue injury, such as heart and brain injuries, are limited in effectiveness, and there is a need for therapies that can limit cell death and restore lost body functions, with the role of B lymphocytes in tissue repair being unclear and previously considered inconsequential.
Innovation Solution
Administration of relatively pure populations of B lymphocytes, including pre-pro-B cells, pro-B cells, immature B cells, and mature B cells, into damaged tissue to enhance recovery, which improves cardiac function and tissue repair by promoting cellular proliferation and reducing apoptosis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional treatments are used for tissue injury, then current standard care is provided, but treatment effectiveness is limited and cell death continues
Solution Approach 1:
The patent uses B lymphocytes as intermediary cells that migrate to injured tissue and mediate repair processes. These B cells serve as a bridge between the immune system and tissue regeneration, secreting cytokines and growth factors that promote healing while reducing harmful inflammation and cell death in the injured myocardium or brain tissue.
2Productivity
If B lymphocytes are administered to injured tissue, then tissue repair is enhanced and cardiac function improves, but the role and mechanism of B cells in tissue repair was previously unclear
Solution Approach 1:
The administered B lymphocytes autonomously migrate to the injured tissue site, self-organize, and initiate repair processes without requiring external direction. The B cells self-regulate their cytokine secretion and interact with other immune cells and tissue components to coordinate the repair process, demonstrating self-service capability in promoting tissue regeneration.
3Reliability
If pure B cell populations are administered, then specific therapeutic effects are achieved, but cell separation and purification complexity increases
Solution Approach 1:
The patent extracts and isolates B lymphocytes from mixed bone marrow or blood cell populations using cell separation techniques based on surface marker expression. This extraction process obtains pure B cell populations while removing other cell types, enabling consistent therapeutic effects by ensuring that only the desired B cells are administered to the patient.
Data Source
AI summary
The present invention relates generally to systems and methods of enhancing recovery of function of injured tissue through administration of a composition comprising a relatively pure populations of B lymphocyte cells in a pharmaceutically acceptable carrier to the injured tissue. Kits are provided to aid in purification of B cells from heterogeneous mixtures of cells and administration of B cells to injured tissue.