B5 Monoclonal Antibody Assays for Specific Glanders Detection
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Solution Overview
Problem
Current laboratory diagnosis of glanders caused by Burkholderia mallei is difficult due to the bacterium's challenge in isolation and non-specific serological tests, which are costly and inefficient for various animal species.
Innovation Solution
Development of B5 monoclonal antibodies with high specificity to Burkholderia mallei lipopolysaccharide (LPS) and their binding fragments, usable in immunoassays like ELISA for detecting B. mallei infections in various animals, with kits and methods providing high sensitivity and specificity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If non-specific serological tests (Haemagglutinin inhibition tests) are used to detect B. mallei infections, then the testing process is simplified, but the specificity and accuracy of detection deteriorates
Solution Approach 1:
The patent changes the key parameter from using non-specific serological tests to using highly specific monoclonal antibodies (B5) that recognize unique epitopes on B. mallei LPS. This parameter change maintains operational simplicity through ELISA format while dramatically improving detection specificity above 95%, resolving the contradiction between ease of operation and measurement precision.
2Adaptability or versatility
If ELISA tests with secondary antibodies against different animal types are developed, then the detection capability for various animals is improved, but the device complexity and cost increases
Solution Approach 1:
The patent applies universality by developing a single ELISA test system using B5 monoclonal antibodies that can detect B. mallei infections across multiple animal species (horses, donkeys, mules, and other mammals) without requiring different secondary antibodies for each species. This universal approach maintains high detection capability while eliminating the complexity of setting up separate test systems for different animals, resolving the contradiction between adaptability and device complexity.
3Measurement precision
If B. mallei isolation and identification procedures are performed, then the diagnostic accuracy is improved, but the time required for diagnosis and the difficulty of the process increases
Solution Approach 1:
The patent applies preliminary action by using B5 monoclonal antibodies that specifically bind to B. mallei LPS epitopes present in clinical specimens. This allows the test to detect the presence of B. mallei directly without requiring time-consuming isolation and identification steps, achieving high diagnostic accuracy while significantly reducing the time required for diagnosis, thus resolving the contradiction between measurement precision and loss of time.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The B5 antibodies achieve sensitivity and specificity above 95% in detecting B. mallei infections, enabling accurate diagnosis and potential therapeutic applications.
Implementation Method 1
B5 monoclonal antibodies with high specificity to Burkholderia mallei lipopolysaccharide (LPS) and their binding fragments, usable in immunoassays like ELISA for detecting B. mallei infections
Data Source
AI summary
Disclosed are B5 hybridoma and a B5 monoclonal antibody to Burkholderia mallei, Burkholderia pseudomallei, or Burkholderia thailandensis and methods of use. Also disclosed are isolated binding fragments, isolated antibody fragments, isolated monoclonal antibodies, isolated chimeric antibodies, and isolated humanized antibodies having the binding fragments of the B5 monoclonal antibody. Also disclosed are assays for detecting B. mallei, B. pseudomallei, or B. thailandensis in a sample, for detecting infection by B. mallei, B. pseudomallei, or B. thailandensis, and therapeutic methods for treating infections by B. mallei, B. pseudomallei, or B. thailandensis.