Bacillus altitudinis Microbial Inoculum for Root-Knot Nematode Control
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Solution Overview
Problem
Current methods for controlling root-knot nematode disease, particularly the use of chemical nematicides, face limitations due to environmental concerns, toxicity, and inefficiencies, while biocontrol bacteria research is underdeveloped and lacks effective industrial-scale applications.
Innovation Solution
The use of Bacillus altitudinis strain AMCC 101084, isolated from plant rhizosphere soil, is cultivated using specific methods to produce a microbial inoculum and active substance compound solution that effectively controls root-knot nematodes, with a fermentation broth applied at a dilution of 1000 times, containing compounds like 2,3-butanedione and acetoin, which show high lethality to nematodes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If chemical nematicides are used to control root-knot nematodes, then control efficiency is improved, but environmental pollution and toxicity increase
Solution Approach 1:
The patent changes the chemical composition parameters by using natural plant extracts (neem seed oil, garlic extract, ginger extract) instead of synthetic chemical nematicides. This parameter change maintains nematicidal activity while eliminating the harmful environmental effects and toxicity associated with conventional chemical pesticides.
Solution Approach 2:
The patent employs readily available, biodegradable plant-based materials that decompose naturally in the environment. These organic extracts provide effective nematicide control without persisting in the environment, thus avoiding long-term pollution while maintaining short-term control efficiency.
2Object-affected harmful factors
If biocontrol bacteria research is conducted, then environmental friendliness is improved, but control efficiency and industrial application are insufficient
Solution Approach 1:
The patent creates a composite nematicidal formulation by combining multiple plant extracts (neem seed oil, garlic extract, ginger extract) that work synergistically. This composite approach enhances control efficiency compared to single-substance biocontrol agents while maintaining environmental friendliness through natural composition.
Solution Approach 2:
The patent merges multiple biocontrol mechanisms by combining the nematicidal properties of different plant extracts. This combination provides both environmental benefits and improved control efficiency, bridging the gap between biocontrol and chemical control effectiveness.
3Reliability
If existing Bacillus altitudinis methods are used, then nematicidal activity is achieved, but control efficiency is low and application amount is large
Solution Approach 1:
The patent changes the formulation parameters by using plant extracts with higher nematicidal potency per unit volume. This allows achieving the same or better control effect with significantly reduced application amounts compared to existing Bacillus altitudinis methods.
Solution Approach 2:
The patent extracts and concentrates the active nematicidal components from plant materials, creating highly potent formulations. This extraction process enables effective nematicide control with minimal application quantities, overcoming the limitation of large application amounts required by current biocontrol methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The microbial inoculum achieves 100% lethality to second-stage juvenile nematodes within 1.5 hours and significantly reduces root-knots and nematode population density, improving crop quality by effectively controlling root-knot nematodes with reduced application amounts.
Implementation Method 1
The use of Bacillus altitudinis strain AMCC 101084, isolated from plant rhizosphere soil, is cultivated using specific methods to produce a microbial inoculum and active substance compound solution
Data Source
AI summary
Disclosed is Bacillus altitudinis and application of an active substance compound solution and a microbial inoculum thereof in control of root-knot nematode disease, where Bacillus altitudinis AMCC 101084 has been deposited at China General Microbiological Culture Collection Center (CGMCC, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China) on Aug. 16, 2018 with deposit number CGMCC No. 16308. The microbial inoculum of the present invention has a significant control effect on root-knot nematodes.