Bacillus amyloliquefaciens ABI01 Spore Formulation for Rhizosphere Persistence
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Solution Overview
Problem
Current Bacillus-based bioformulations for plant protection have limited shelf life and effectiveness due to short persistence in the plant rhizosphere, and insufficient knowledge of their active components, which restricts their long-term efficacy against fungal diseases.
Innovation Solution
Development of Bacillus amyloliquefaciens subsp. plantarum strain ABI01, which exhibits enhanced persistence and antifungal performance, producing a range of antimicrobial compounds and inducing systemic resistance in plants, with a focus on the strain's genome sequence and secondary metabolites like amylocyclicin and difficidin for improved biocontrol capabilities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If Bacillus-based bioformulations are used for plant protection, then environmental friendliness and biocontrol capability are improved, but shelf life and persistence in the plant rhizosphere are limited
Solution Approach 1:
The patent applies parameter changes by optimizing culture conditions (temperature, pH, aeration, nutrient composition) and formulation parameters (spore concentration, carrier materials, stabilizers) to enhance both the stability during storage and the persistence in the rhizosphere. This resolves the contradiction by adjusting physical and chemical parameters of the bioformulation system.
2Duration of action of moving object
If current Bacillus strains are used, then short-term antifungal effect is achieved, but long-term efficacy is limited due to insufficient persistence
Solution Approach 1:
The patent employs composite materials by formulating Bacillus amyloliquefaciens spores with specific carrier materials and protective agents that enhance both shelf life and rhizosphere persistence. The composite formulation includes spores, carriers, and stabilizers that work synergistically to extend the duration of antifungal activity while maintaining reliability in the plant root zone.
3Device complexity
If knowledge of active components is insufficient, then formulation development is simplified, but biocontrol efficacy is reduced
Solution Approach 1:
The patent uses secondary metabolites produced by Bacillus amyloliquefaciens as intermediaries that mediate the biocontrol effect. These metabolites (such as iturin, fengycin, and bacillomycin) serve as the active components that directly inhibit fungal pathogens, bridging the gap between the bacterial strain and the desired biocontrol efficacy without requiring complete understanding of all molecular mechanisms.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
ABI01 demonstrates superior antifungal activity compared to existing bio- and chemofungicides, providing long-lasting protection against pathogens like Rhizoctonia solani, with increased persistence in the plant root zone and enhanced biocontrol efficacy.
Implementation Method 1
ABI01 demonstrates superior antifungal activity compared to existing bio- and chemofungicides, providing long-lasting protection against pathogens like Rhizoctonia solani
Implementation Method 2
producing a range of antimicrobial compounds and inducing systemic resistance in plants, with a focus on the strain's genome sequence and secondary metabolites like amylocyclicin and difficidin for improved biocontrol capabilities
Implementation Method 3
ABI01 demonstrates superior antifungal activity compared to existing bio- and chemofungicides, providing long-lasting protection against pathogens like Rhizoctonia solani, with increased persistence in the plant root zone
Data Source
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AI summary
The invention addresses the problem of developing an environmentally compatible, storable and long-acting agent against phytopathogenic microorganisms, especially fungi, on the basis of defined biological factors. Said problem is solved by an antifungal agent for treating fungal and other microbial and viral infections, which is prepared on the basis of spores of Bacillus amyloliquefaciens plantarum. Representatives of said taxonomic group have the ability to form at least ten different antimicrobial substances which belong to the families of dipeptides (bacilysin), lipopeptides and siderophores (bacillomycin D, fengycin, surfactin and bacillibactin), polyketides (difficidin, bacillaene and macrolactin) and the group consisting of bacteriocins/microcins (amylocyclicin and plantazolicin). The new strain B. amyloliquefaciens plantarum AB101 is characterised by improved persistence in the area of the plant rhizospere and increased effectiveness against the root rot disease ("black scurf") in potatoes caused by Rhizoctonia solani. The bacteriocin amylocyclicin produced from AB101 is a previously unknown bacteriocin having a strong effect against gram-positive bacteria. The invention can be used in plant protection, biotechnology and agriculture.