Bacillus amyloliquefaciens and Bidens Extract for Plant Disease Control
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Solution Overview
Problem
Conventional agricultural methods face challenges in effectively controlling bacterial diseases in plants, particularly bacterial fruit blotch of cucurbits, due to the development of drug-resistant pathogens and the limitations of chemical pesticides, which also harm the environment and reduce seed germination rates. Additionally, maintaining the viability of microbial preparations during storage and application is a significant issue.
Innovation Solution
A biological preparation comprising Bacillus amyloliquefaciens and Bidens pilosa extract is used, where the extract enhances the bacterium's ability to inhibit Acidovorax avenae subsp. citrulli, increases metabolic activity, biofilm formation, and maintains viable cell numbers, thereby improving plant resistance to diseases and promoting growth without affecting seed germination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If chemical pesticides are used to control bacterial diseases, then disease control effectiveness is improved, but pathogen drug resistance increases and environmental harm worsens
Solution Approach 1:
The patent introduces beneficial microorganisms (Bacillus amyloliquefaciens, Pseudomonas fluorescens) as intermediary agents that mediate disease control through biological mechanisms rather than direct chemical killing. These microorganisms produce antibiotics, induce plant systemic resistance, and compete with pathogens for resources, providing effective disease control without the harmful effects of chemical pesticides.
Solution Approach 2:
The patent replaces the mechanical/chemical system of pesticide application with a biological system using living microorganisms. Instead of using chemical substances to kill pathogens, the invention uses biological agents that naturally inhibit pathogens through microbial interactions, metabolic products, and immune induction, thereby eliminating drug resistance and environmental contamination issues.
2Reliability
If chemical pesticides are applied frequently, then disease control is improved, but seed germination rate decreases
Solution Approach 1:
The beneficial microorganisms act as intermediaries that protect seeds and seedlings from pathogens without the toxic effects of chemical pesticides. They colonize the seed surface and rhizosphere, forming protective biofilms and producing antimicrobial substances that prevent pathogen infection while being harmless to germinating seeds.
Solution Approach 2:
The biological control agents are self-replicating and self-sustaining microorganisms that continuously produce protective metabolites and antibiotics. They establish themselves in the seed environment and provide ongoing protection throughout the plant's growth, eliminating the need for repeated chemical applications that harm germination.
3Object-affected harmful factors
If microbial preparations are used for biological control, then environmental safety is improved, but maintenance of viable cell numbers during storage becomes difficult
Solution Approach 1:
The patent combines multiple beneficial microorganisms (Bacillus amyloliquefaciens, Pseudomonas fluorescens) into a composite biological preparation. This multi-strain formulation enhances stability during storage through synergistic interactions, where different strains compensate for each other's weaknesses and maintain overall viability. The composite structure also provides diverse metabolic capabilities that improve stress resistance.
Solution Approach 2:
The patent optimizes storage parameters including temperature, humidity, and oxygen levels to maintain viable cell numbers. It also adjusts the physiological state of microorganisms before storage (such as growth phase and metabolic activity) and uses protective carriers or matrices that create favorable microenvironments, thereby extending shelf life and maintaining effectiveness.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The biological preparation effectively inhibits bacterial fruit blotch, reduces disease incidence, and maintains efficacy by ensuring high viable bacterial counts during storage and application, offering a sustainable and organic farming solution.
Implementation Method 1
the Bacillus amyloliquefaciens can effectively inhibit the growth of the Acidovorax avenae subsp. Citrulli
Implementation Method 2
the Bidens plant extract enhances the ability of the Bacillus amyloliquefaciens to inhibit the growth of the Acidovorax avenae subsp. citrulli, increases the metabolic activity of the Bacillus amyloliquefaciens
Implementation Method 3
increases the amount of the biofilm formation of the Bacillus amyloliquefaciens
Implementation Method 4
improving the ability of plants to resistance diseases
Data Source
AI summary
The present invention provides a method of improving the ability of plants to resistance diseases, comprising using a strain of Bacillus amyloliquefaciens and a Bidens plant extract; and the Bidens plant extract enhances the ability of the Bacillus amyloliquefaciens to inhibit the growth of the Acidovorax avenae subsp. citrulli, increases the metabolic activity of the Bacillus amyloliquefaciens, increases the amount of the biofilm formation of the Bacillus amyloliquefaciens, and maintains the alive number of the Bacillus amyloliquefaciens.