Bionematicide Composition Using Bacillus Strains for Nematode Control

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Solution Overview

Problem

Current methods for controlling phytopathogenic nematodes in agriculture are often ineffective and environmentally harmful, with limited availability of commercially viable biopesticides due to inconsistent results in mass production and application.

Innovation Solution

A bionematicide composition using isolated Bacillus cereus, Bacillus thuringiensis, and Bacillus thuringiensis strains from Chilean soil, combined with agronomically acceptable carriers, is developed to control nematodes without conventional chemical insecticides, available in various formulations for application on plants or seeds.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical nematicides are used to control nematode populations, then nematode control effectiveness is improved, but environmental harm and negative impact on beneficial organisms increases

Engineering Contradiction:

Improvenematode control effectivenessVSAvoidenvironmental harm

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent utilizes naturally occurring microorganisms (bacteria, fungi, viruses) that exist in the environment to control nematodes. These native biocontrol agents are applied to enhance natural predation and parasitism, converting the existing ecological relationships into beneficial control mechanisms without introducing harmful chemicals.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent employs microorganisms as intermediary agents between the application and nematode control. These biocontrol agents act as mediators that naturally infect, parasitize, or predation on nematodes, providing control effectiveness while avoiding direct chemical harm to the environment and beneficial organisms.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Object-affected harmful factors

If biopesticides are used as biological control agents, then environmental friendliness is improved, but commercial availability and consistency of results worsens due to mass production difficulties

Engineering Contradiction:

Improveenvironmental friendlinessVSAvoidmass production consistency

Core Design Contradiction:

Object-affected harmful factorsVSEase of manufacture

Solution Approach 1:

The patent employs multiple microorganisms from different biological groups (bacteria, fungi, viruses) that can be applied together or separately. This multi-functional approach ensures that at least some of the biocontrol agents will be effective under various environmental conditions, improving consistency of results while maintaining environmental friendliness.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Solution Approach 2:

The patent creates composite biopesticide formulations containing multiple types of microorganisms. By combining different biocontrol agents with complementary modes of action, the formulation achieves more consistent and reliable nematode control compared to single-agent products, while maintaining the environmental benefits of biological control.

Inventive Principle:

Principle #40Composite materials

3Reliability

If conventional chemical insecticides are used for nematode control, then nematode population reduction is improved, but plant safety and user toxicity increases

Engineering Contradiction:

Improvenematode population reductionVSAvoidtoxicity to plants and users

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent converts the use of harmful chemical substances into beneficial biological control by employing microorganisms that naturally occur in the environment. These biocontrol agents provide nematode population reduction through natural infection and parasitism mechanisms, eliminating the need for toxic chemical insecticides.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The microorganisms used in the patent are self-replicating and self-sustaining biological agents. Once applied, they can reproduce and maintain their population in the environment, providing ongoing nematode control without requiring repeated applications of toxic chemicals, thereby reducing both plant safety risks and user exposure to harmful substances.

Inventive Principle:

Principle #25Self-service

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The bionematicide composition effectively reduces nematode populations and promotes plant growth, outperforming commercial products in controlled and field conditions, with no environmental restrictions, suitable for conventional and organic agricultural use.

Implementation Method 1

at least one strain belonging to a group of bacterial strains isolated from Chilean soil, and belonging to the genus Bacillus specifically Bacillus cereus bacteria Peumo strain... Bacillus cereus Bromelia strain... and Bacillus thuringiensis Anemophyla strain... The bionematicide composition effectively reduces nematode populations

Methodology Applied

Scientific EffectNematicidal activity:

Data Source

PatentUS9468215B2Bionematicide composition and method for controlling phytopathogenic nematodes using the same

Publication Date: 2016.10.18 BIO INSUMOS NATIVA SPA

AI summary

The present invention relates to the field of biotechnology, and particularly relates to a bionematicide composition broad spectrum action on phytopathogenic nematodes which comprises one or more bacterial strains isolated from Chilean soil belonging to the genus Bacillus, and the method of use of this composition for the protection of plants against nematode attack.