Bacillus Carrier Biofertilizer for Phosphate Solubilization in Alkaline Soils

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Solution Overview

Problem

Conventional phosphate fertilizers cause soil acidification, nutrient imbalances, and microbial inhibition in alkaline soils, limiting phosphate solubilization and agricultural productivity.

Innovation Solution

A carrier-based agricultural biofertilizer composition using the Bacillus megaterium strain CGAPGPBBS-034, isolated from alkaline calcareous soil, which produces phytohormones and siderophores, enhancing phosphate solubilization and plant growth in higher pH environments.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If conventional phosphate fertilizers are applied to improve phosphate availability, then plant growth is promoted, but soil acidification occurs and microbial activity is reduced

Engineering Contradiction:
Improvephosphate availabilityVSAvoidsoil acidification and microbial inhibition
Core Design Contradiction:
Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent uses phosphate-solubilizing bacteria (PSB) as an intermediary organism to convert insoluble phosphate into soluble forms that plants can absorb. The PSB produce organic acids and enzymes that dissolve phosphate minerals, providing a biological mediation approach between unavailable phosphate and plant uptake needs, avoiding the direct application of acidic chemical fertilizers

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The invention replaces the chemical mechanism of phosphate fertilizers with a biological mechanism. Instead of directly applying soluble phosphate salts that acidify soil, the system employs living bacteria that naturally solubilize phosphate through metabolic processes, substituting chemical input with biological functionality

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Quantity of substance

If conventional phosphate fertilizers are used to increase phosphate levels, then nutrient availability improves, but nutrient imbalances and salinity increase

Engineering Contradiction:
Improvephosphate concentrationVSAvoidnutrient imbalance and salinity
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The phosphate-solubilizing bacteria perform self-service by utilizing carbon sources and energy from their metabolism to produce organic acids that solubilize phosphate. The system is self-regulating, with bacteria activity naturally responding to phosphate availability and plant needs, avoiding the over-application and accumulation problems associated with chemical fertilizer use

Inventive Principle:
Principle #25Self-service

3Productivity

If phosphate fertilizers are applied to enhance phosphate solubilization, then plant productivity increases, but environmental pollution occurs

Engineering Contradiction:
Improvecrop yieldVSAvoidgroundwater and surface water pollution
Core Design Contradiction:
ProductivityVSObject-generated harmful factors

Solution Approach 1:

The invention converts the problem of insoluble phosphate (which plants cannot utilize) into a benefit by employing bacteria that transform it into soluble forms. This approach utilizes the previously wasted phosphate reserves in soil and rock, converting an inert substance into a biologically available nutrient without introducing external chemical pollutants

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

4Productivity

If microbial activity is stimulated to improve phosphate solubilization, then nutrient cycling enhances, but microbial viability is reduced in alkaline soils

Engineering Contradiction:
Improvephosphate solubilization rateVSAvoidmicrobial stability and viability
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent employs carrier materials with specific physical and chemical properties (porosity, surface area, pH buffering capacity) that create a favorable microenvironment for bacterial survival in alkaline soils. The carrier modifies local conditions around the bacteria, protecting them from adverse pH and temperature fluctuations while maintaining their solubilization activity

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The biofertilizer composition effectively solubilizes phosphate in alkaline soils, improving plant growth, germination rates, and overall health, while maintaining bacterial stability and viability for extended periods.

Implementation Method 1

These beneficial microorganisms possess the ability to solubilize inorganic phosphorus, converting it into soluble forms that plants can readily absorb. By releasing organic acids and enzymes, PSB break down insoluble phosphate, making it accessible to plant roots.

Methodology Applied
Scientific EffectPhosphate solubilization: Chemical Bonding

Implementation Method 2

A carrier-based agricultural biofertilizer composition using the Bacillus megaterium strain CGAPGPBBS-034, isolated from alkaline calcareous soil, which produces phytohormones and siderophores, enhancing phosphate solubilization and plant growth in higher pH environments.

Methodology Applied
Scientific EffectPhytohormone production: Chemical Bonding

Implementation Method 3

A carrier-based agricultural biofertilizer composition using the Bacillus megaterium strain CGAPGPBBS-034, isolated from alkaline calcareous soil, which produces phytohormones and siderophores, enhancing phosphate solubilization and plant growth in higher pH environments.

Methodology Applied
Scientific EffectSiderophore production: Chemical Bonding

Implementation Method 4

an agriculturally acceptable liquid stabilizer selected to provide stability and support for the Bacillus strain, thereby enhancing the growth and overall health of the plants

Methodology Applied
Scientific EffectAdsorption: Adsorption

Data Source

PatentUS20250214908A1Carrier-based agricultural biofertilizer composition and method of using the same
Publication Date: 2025.07.03 BERRY BENOY
  • US20250214908A1 patent drawing

AI summary

Exemplary embodiments of the present disclosure are directed towards a carrier-based agricultural biofertilizer composition, comprising an isolated Bacillus megaterium strain CGAPGPBBS-034 having the deposit accession number KY495205; and an agriculturally acceptable liquid formulation with a stabilizer to provide stability and support for at least one of the Bacillus species, thereby enhancing the growth and overall health of the plants.