Bacillus coagulans spore surface modification for antiviral efficacy
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Solution Overview
Problem
Current probiotic compositions lack significant prophylactic effects against respiratory viruses, particularly influenza, due to the absence of covalently chemically modified spores of Bacillus coagulans, which limits their immunomodulatory and antiviral efficacy.
Innovation Solution
Incorporating covalently chemically modified spores of Bacillus coagulans with varying degrees of surface modification, along with compounds like benzylisoquinoline derivatives, gibberellin, para-aminobenzoic acid, carnitine, and auxin or its derivatives, to enhance adhesion and germination, thereby increasing immunomodulatory and antiviral effects in food, cosmetic, and pharmaceutical compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional probiotics are used, then gastrointestinal health is improved, but prophylactic effect against respiratory viruses is insufficient
Solution Approach 1:
The patent applies parameter changes by covalently chemically modifying the spores of Bacillus coagulans with varying degrees of surface modification. This chemical modification alters the physical and chemical parameters of the spore surface, enhancing adhesion properties and germination efficiency, thereby significantly improving immunomodulatory and antiviral effects compared to traditional unmodified probiotics.
Solution Approach 2:
The patent creates a composite probiotic system by combining covalently chemically modified spores of Bacillus coagulans with specific compounds including benzylisoquinoline derivatives, gibberellin, para-aminobenzoic acid, carnitine, and auxin or its derivatives. This composite formulation synergistically enhances adhesion, germination, and immune response stimulation, providing superior prophylactic protection against respiratory viruses.
2Reliability
If spore-forming bacteria are used, then resistance to technological processes and storage is improved, but adhesion and germination efficiency is insufficient
Solution Approach 1:
The patent modifies the spore surface parameters through covalent chemical modification, which maintains the inherent resistance of spore-forming bacteria to technological processes and storage while significantly enhancing adhesion properties and germination efficiency. The chemical modification alters surface characteristics without compromising spore durability.
Solution Approach 2:
The patent introduces specific compounds as intermediaries that facilitate spore germination and adhesion. These compounds including gibberellin, auxin, and other bioactive substances act as mediators between the modified spores and the host system, enhancing germination efficiency and adhesion to epithelial cells while preserving spore resistance properties.
3Adaptability or versatility
If probiotics are incorporated into food and cosmetic products, then versatility is improved, but stability and efficacy is insufficient
Solution Approach 1:
The patent creates a stable composite formulation suitable for diverse applications by combining covalently chemically modified spores with multiple stabilizing compounds. This composite structure maintains probiotic viability and efficacy across different product types including food, cosmetic, and pharmaceutical formulations, enabling broad versatility without compromising stability.
Solution Approach 2:
The patent optimizes formulation parameters by incorporating specific compounds that enhance stability under various storage and application conditions. The covalent chemical modification of spores and the inclusion of stabilizing agents allow the probiotic to maintain its antiviral efficacy across different product matrices and environmental conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified spores demonstrate enhanced adhesion, germination, and immune response stimulation, leading to improved immunomodulatory and antiviral effects, including protection against influenza, rotaviruses, and coronaviruses, with rapid onset and stability in various product forms.
Implementation Method 1
enhanced adhesion, germination, and immune response stimulation
Implementation Method 2
spores of Bacillus coagulans can germinate in vegetative bacteria in the lumen of the human intestine and exert their probiotic effects
Implementation Method 3
The metabolites possess an immunomodulatory effect, the metabolites secreted by the bacterium by the cell wall and spores of bacteria
Implementation Method 4
Bacillus coagulans produce coagulin—a bacteriocin belonging to the pediocin family... This bacteriocin is encoded by an approximately 14 kB fragment in plasmid pI4
Data Source
AI summary
Field of application: the invention relates to biotechnology, can be used in the food industry, medicine and cosmetology in the prevention and/or treatment of diseases associated with immunodeficiencies and respiratory viral infections.The proposed food, cosmetic and pharmaceutical composition with an immunomodulating and protective antiviral effect additionally has the ability to completely prevent infection by a number of viruses, including influenza, rotavirus and coronavirus, as well as stimulate the immune system more than individual probiotics.