Bacillus Gibsonii Protease Substitutions for Washing Catalytic Activity

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Solution Overview

Problem

Existing proteases in detergents and cleaning agents do not exhibit sufficient catalytic performance under standard washing conditions, particularly when dealing with protein-containing soils.

Innovation Solution

Amino acid substitutions at positions 211 and 212 of the Bacillus gibsonii protease, specifically M211N and P212D or M211L and P212D, enhance proteolytic activity, resulting in improved catalytic performance under standard washing conditions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If wild-type protease from Bacillus gibsonii is used, then the structure is simple and production is easy, but the catalytic performance under standard washing conditions is insufficient

Engineering Contradiction:
Improvecatalytic performanceVSAvoidamino acid sequence complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies parameter changes by modifying specific amino acid positions (211 and 212) in the protease sequence. The substitutions M211N/P212D or M211L/P212D change the chemical properties at these positions, improving catalytic performance while maintaining overall sequence simplicity and ease of production through targeted, minimal modifications

Inventive Principle:
Principle #35Parameter changes

2Productivity

If proteases are modified to improve catalytic activity, then the cleaning performance improves, but the production complexity increases

Engineering Contradiction:
Improvecleaning performanceVSAvoidproduction ease
Core Design Contradiction:
ProductivityVSEase of manufacture

Solution Approach 1:

The patent applies local quality by making targeted amino acid substitutions only at positions 211 and 212 of the protease sequence. This localized modification approach improves cleaning performance through enhanced catalytic activity at specific sites while keeping the overall production process relatively simple, as only two positions require modification rather than comprehensive sequence redesign

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The modified proteases demonstrate increased catalytic activity, achieving at least 110% of the wild-type variant's performance, effectively removing protein-containing soils in a temperature range of 40°C to 60°C.

Implementation Method 1

They act as nonspecific endopeptidases and hydrolyze any acid amide bonds found within peptides or proteins

Methodology Applied
Scientific EffectProteolytic activity: Hydrolysis

Implementation Method 2

The modified proteases demonstrate increased catalytic activity, achieving at least 110% of the wild-type variant's performance

Methodology Applied
Scientific EffectEnzyme catalysis: Catalysis

Data Source

PatentEP3472312B1Protease from bacilllus gibsonii and variants thereof
Publication Date: 2025.08.20 HENKEL KGAA

AI summary

The invention relates to proteases comprising an amino acid sequence which has at least 70% sequence identity to the amino acid sequence given in SEQ ID No. 1 over its entire length, and which has an amino acid substitution on at least one of the positions corresponding to the positions 12, 43, 122, 127, 154, 156, 160, 211 or 222, relating in each case to the numbering according to SEQ ID No. 1. The invention also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.