Bacillus sp. 2-4 Probiotic Feed Additive for Aquaculture
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Solution Overview
Problem
Current probiotics for fish are often species-specific, unstable, and imported strains may not effectively address disease prevention in aquaculture due to low colony forming units and lack of adaptation to local environments, raising concerns about their efficacy and availability.
Innovation Solution
An isolated Bacillus sp. 2-4 strain, specifically selected for its antibacterial properties and stability, is used as a live bacterial preparation for fish and crustaceans, formulated into feed additives to enhance disease resistance and growth promotion.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If Lactobacillus is used as probiotics for fish, then disease prevention effect is improved, but species-specificity limits applicability to different fish species
Solution Approach 1:
The patent isolates and utilizes Bacillus subtilis strain, a universal probiotic strain that can be applied across multiple fish species rather than being limited to a single species. This strain demonstrates broad-spectrum antibacterial activity against various fish pathogens while maintaining compatibility with different aquatic environments, thereby achieving multi-functionality and versatility across species.
2Adaptability or versatility
If imported probiotic strains are used, then availability is improved, but stability and colony forming unit are reduced due to time lag
Solution Approach 1:
The patent establishes local isolation and cultivation of Bacillus subtilis strains within Korea, performing preliminary preparation of stable, adapted probiotic cultures before they are needed. This eliminates the time lag associated with importing strains, ensuring high colony forming units and stability upon domestic distribution and application.
3Productivity
If probiotics are administered to prevent diseases, then growth rate is improved, but antibiotic use restriction creates limitation
Solution Approach 1:
The patent converts the restriction on antibiotic use into an opportunity to develop and utilize native Bacillus subtilis probiotic strains. These naturally derived probiotics provide disease prevention and growth promotion effects without relying on antibiotics, thereby benefiting from the regulatory restriction while maintaining productivity improvements.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Bacillus sp. 2-4 strain effectively prevents massive death from pathogens in farm-raised fish and crustaceans by exhibiting strong antibacterial activity against key fish pathogens and promoting digestive health without affecting the hatch or vitality of fish and their feeds.
Implementation Method 1
The Bacillus sp. 2-4 strain effectively prevents massive death from pathogens in farm-raised fish and crustaceans by exhibiting strong antibacterial activity against key fish pathogens
Implementation Method 2
probiotics play a role in preventing the growth of harmful bacteria by lowering the pH inside the intestine through production of lactic acids
Implementation Method 3
the administrated live bacterial preparation produces bacteriocin or peroxides to suppress the proliferation of pathogens
Data Source
AI summary
The present invention relates to an isolated Bacillus sp. strain having probiotic activity. More particularly, the present invention relates to an isolated Bacillus sp. 2-4 (KCCM11107P) strain having probiotic activity. Since the strain of the present invention functions as probiotics by having an antibacterial activity against various fish pathogenic bacteria, as well as secreting helpful enzymes such as protease, amylase, cellulose, and lipase to help the digestion and absorption of the feed, the strain of the present invention can be helpfully used as a feed additive for fish and crustaceans.