Bacillus Probiotic Feed for Shrimp AHPND and WSS Control

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Solution Overview

Problem

Current shrimp farming faces significant challenges in preventing and treating acute hepatopancreatic necrosis disease (AHPND) and white spot syndrome (WSS) due to the rapid spread of Vibrio parahaemolyticus and WSSV, which cause high mortality rates and require effective, safe, and sustainable solutions.

Innovation Solution

A feed composition containing Bacillus subtilis, Bacillus pumilus, and Bacillus licheniformis strains is developed, which exhibits antibacterial activity against Vibrio parahaemolyticus and antiviral activity against WSSV, improving shrimp immunity and water quality, thereby preventing and treating AHPND and WSS.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibiotics are used to treat pathogenic bacteria in shrimp farming, then disease treatment effectiveness is improved, but safety and regulatory compliance deteriorate

Engineering Contradiction:

Improvedisease treatment effectivenessVSAvoidsafety and regulatory compliance

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful role of bacteria by using beneficial Bacillus species (B. subtilis, B. pumilus, B. licheniformis) that produce antimicrobial substances to combat pathogenic bacteria. This transforms the approach from directly killing pathogens with antibiotics to using competitive microbial mechanisms, achieving disease control without antibiotic safety concerns

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent introduces Bacillus probiotics as intermediary organisms that mediate the control of pathogenic bacteria. These probiotics produce antimicrobial substances and compete for resources, acting as a safe intermediary that eliminates the need for direct antibiotic application to shrimp

Inventive Principle:

Principle #24Intermediary (Mediator)

2Object-affected harmful factors

If probiotics are used to prevent disease in shrimp, then safety is improved, but immunoactivity and disease prevention effectiveness deteriorate

Engineering Contradiction:

ImprovesafetyVSAvoidimmunoactivity and disease prevention effectiveness

Core Design Contradiction:

Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent creates a composite probiotic formulation containing three different Bacillus species (B. subtilis, B. pumilus, B. licheniformis) with complementary functions. Each species contributes different antimicrobial mechanisms and immune-stimulating properties, creating a synergistic effect that enhances overall immunoactivity while maintaining safety

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

The patent optimizes parameters including the specific strain selections, their ratios in the formulation, and cultivation conditions to maximize probiotic effectiveness. By adjusting these parameters, the formulation achieves enhanced immunoactivity and disease prevention capability while maintaining the safety benefits of probiotics

Inventive Principle:

Principle #35Parameter changes

3Speed

If Vibrio parahaemolyticus spreads rapidly in shrimp farming, then disease transmission speed is increased, but control and management difficulty increases

Engineering Contradiction:

Improvedisease transmission speedVSAvoidcontrol and management difficulty

Core Design Contradiction:

SpeedVSDevice complexity

Solution Approach 1:

The patent applies probiotics preliminarily to shrimp feed before disease outbreak occurs. The Bacillus probiotics are administered in advance to establish protective mechanisms in shrimp, including immune stimulation and pathogen inhibition, so that when Vibrio parahaemolyticus attempts to infect, the shrimp are already protected

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent utilizes the natural antimicrobial substances produced by Bacillus species to counteract the harmful effects of rapid Vibrio spread. By harnessing these natural defensive mechanisms, the system converts the threat of rapid disease transmission into an opportunity for proactive biological control

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The feed composition significantly enhances shrimp survival rates, growth rates, and non-specific immunity, reducing toxin production in hepatopancreas and improving water quality, making it an effective solution for AHPND and WSS prevention and treatment in shrimp farming.

Implementation Method 1

the composition comprising a Bacillus subtilis strain, a Bacillus pumilus strain, and a Bacillus licheniformis strain has antibacterial activity against Vibrio parahaemolyticus

Methodology Applied

Scientific EffectAntibacterial activity:

Implementation Method 2

the composition comprising a Bacillus subtilis strain, a Bacillus pumilus strain, and a Bacillus licheniformis strain has antiviral activity against white spot syndrome virus

Methodology Applied

Scientific EffectAntiviral activity:

Implementation Method 3

the composition comprising a Bacillus subtilis strain, a Bacillus pumilus strain, and a Bacillus licheniformis strain exhibits an effect of improving immunity of the shrimp hepatopancreas

Methodology Applied

Scientific EffectImmune stimulation:

Data Source

PatentUS11432566B2Feed composition for preventing or treating acute hepatopancreatic necrosis disease (AHPND) or white spot syndrome (WSS), comprising a Bacillus subtilis strain, a Bacillus pumilus strain, and a Bacillus licheniformis strain as active ingredients

Publication Date: 2022.09.06 CJ CHEILJEDANG CORP

AI summary

A feed composition for preventing or treating acute hepatopancreatic necrosis disease (AHPND) or white spot syndrome (WSS), having a Bacillus subtilis (KCCM11143P) strain, a Bacillus pumilus (KCCM11144P) strain, and a Bacillus licheniformis (KCCM11270P) strain; culture media thereof; concentrates thereof; or dry matters thereof as an active ingredient. The feed composition exhibits antibacterial activity against Vibrio parahaemolyticus, which causes shrimp AHPND, and antiviral activity against white spot syndrome virus (WSSV), which causes WSS.