Bacillus Spore Preservation Using Metal Oxide Coatings
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current microbial pesticidal compositions face challenges in preserving viable spores of bacteria like Bacillus for long periods due to issues such as rapid cell death and contamination, especially when handling large quantities, and existing methods are not suitable for large-scale applications.
Innovation Solution
A microbial pesticidal composition is developed using a blend of viable spores of Bacillus subtilis and/or Bacillus amyloliquefaciens combined with metal oxides, hydroxides, or their silicic acid salts, where the spores are encapsulated within micro granules or granules with a surface coating of these materials, maintaining stability and viability over time.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If general preservation methods (freeze-drying, paraffin oil storage, slant medium) are used for microorganisms, then small-scale amounts can be preserved, but large-scale handling and long-term stability are not achieved
Solution Approach 1:
The invention changes the physical and chemical parameters of the preservation environment by using supercritical carbon dioxide extraction to remove lipids and oils from the carrier material. This parameter change (removing organic components that support microbial growth) enables long-term preservation of large quantities of microorganisms without contamination or degradation, resolving the contradiction between quantity and preservation duration.
2Reliability
If substrate with nutrient components is used for microbial formulation, then microorganisms can be supported, but pathogenic microbes grow during preservation
Solution Approach 1:
The invention extracts and removes lipid and oil components from the carrier substrate using supercritical carbon dioxide extraction. By taking out these nutrient components that support microbial growth, the formulation can maintain microorganisms without providing food for pathogenic contamination, thus resolving the contradiction between microbial support and prevention of pathogenic growth.
3Duration of action of stationary object
If adsorbent with ammonia-adsorbing ability is mixed in formulation, then some preservation effect is achieved, but water addition deactivates the adsorbent and long-term stability is not obtained
Solution Approach 1:
The invention changes the chemical composition parameters of the carrier material by removing lipids and oils through supercritical CO2 extraction. This creates an inert, water-compatible substrate that provides preservation stability without deactivating when water is added during formulation, resolving both the preservation duration and manufacturing ease contradictions.
4Reliability
If chemical fungicide is mixed with bacterial spores, then disease control is enhanced, but spore viability decreases
Solution Approach 1:
The invention uses a lipid-free carrier material as an intermediary substance between the bacterial spores and chemical fungicide. This intermediary protects the spores from direct contact and deactivating effects of the chemical fungicide while still allowing the fungicide to provide disease control, thus resolving the contradiction between disease control effectiveness and spore viability maintenance.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach ensures the long-term preservation of viable spores in a living state, maintaining their effectiveness and stability even after storage for several years, without significant loss of viability.
Implementation Method 1
a microbial pesticidal composition comprising a blend of viable spores of Bacillus subtilis and/or Bacillus amyloliquefaciens and at least one member selected from a metal oxide, a metal hydroxide, a metal oxide silicic acid salt, and a metal hydroxide silicic acid salt
Data Source
AI summary
[Problem]A microbial pesticidal composition containing viable spores of a bacterium belonging to the genus Bacillus and a chemical pesticide, in which the spores can be preserved stably in a living state for a long period of time, and a method for preserving the spores stably in a living state for a long period of time, are provided.[Dissolution Means]Viable spores of Bacillus subtilis and/or Bacillus amyloliquefaciens are blended with at least one member selected from a metal oxide, a metal hydroxide, a metal oxide silicic acid salt, and a metal hydroxide silicic acid salt.