Bacillus Megaterium Strain for Soil Microbiome and Crop Growth

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Solution Overview

Problem

The overuse of organic and chemical fertilizers leads to soil contamination, imbalance of soil nutrients, pest outbreaks, and decreased farm incomes, contradicting sustainable agriculture principles and causing environmental issues, while current microbial fertilizers lack validation of their beneficial activity on crops.

Innovation Solution

A novel Bacillus megaterium strain, HyangYak-01, is isolated and used to create a microbial agent that improves soil microbiome diversity, physiochemical properties, and promotes crop growth, biosynthesis of biologically active substances, and is applied in cosmetic and pharmaceutical compositions for skin condition improvement.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If chemical fertilizers are used to maintain higher yields, then crop productivity is improved, but soil contamination and environmental issues worsen

Engineering Contradiction:
Improvecrop yieldVSAvoidsoil contamination
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent replaces chemical fertilizers with a biological system - specifically Bacillus megaterium HyangYak-01 strain that produces natural plant growth-promoting substances. This substitution eliminates chemical contamination while maintaining crop productivity through biological mechanisms such as nitrogen fixation, phosphate solubilization, and production of indole-3-acetic acid.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The Bacillus megaterium strain serves itself by naturally producing and secreting plant growth-promoting substances (indole-3-acetic acid, gibberellin, cytokinin) that benefit the host plant. The bacteria establish symbiotic relationships with plant roots, continuously providing nutrients and growth factors without requiring external chemical inputs.

Inventive Principle:
Principle #25Self-service

2Object-affected harmful factors

If microbial fertilizers are used as alternatives to chemical fertilizers, then environmental safety is improved, but validation of beneficial activity on crops is insufficient

Engineering Contradiction:
Improveenvironmental safetyVSAvoidvalidation of beneficial activity
Core Design Contradiction:
Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent implements comprehensive feedback mechanisms to validate the beneficial activity of the Bacillus megaterium strain. Multiple assays are performed including measurement of plant growth parameters (height, root length, biomass), analysis of plant physiological responses (chlorophyll content, photosynthetic rate), and verification of microbial activities (nitrogen fixation, phosphate solubilization). This multi-parameter feedback system provides reliable validation of the strain's effectiveness.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent systematically measures and compares multiple parameters between treated and control groups to validate beneficial activity. These include quantitative parameters (plant height, root length, biomass weight, chlorophyll content) and qualitative parameters (microbiome diversity, soil physicochemical properties). The consistent improvement across multiple parameters provides robust validation of the strain's effectiveness.

Inventive Principle:
Principle #35Parameter changes

3Object-affected harmful factors

If chemical pesticides and fertilizers are reduced, then ecosystem safety is improved, but the number of available agricultural products decreases

Engineering Contradiction:
Improveecosystem safetyVSAvoidnumber of agricultural products
Core Design Contradiction:
Object-affected harmful factorsVSQuantity of substance

Solution Approach 1:

The Bacillus megaterium HyangYak-01 strain performs multiple functions simultaneously: it acts as a fertilizer by fixing nitrogen and solubilizing phosphate, as a plant growth promoter by producing hormones, and as a biocontrol agent by suppressing soil-borne pathogens. This multi-functionality replaces multiple chemical products (fertilizers, pesticides, growth regulators) with a single biological agent, maintaining agricultural productivity while improving ecosystem safety.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Data Source

PatentEP4692310A1Novel bacillus megaterium hyangyak-01 strain and uses thereof
Publication Date: 2026.02.11 COSMAX INC
  • EP4692310A1 patent drawingFigure 1
  • EP4692310A1 patent drawingFigure 2A
  • EP4692310A1 patent drawingFigure 2B

AI summary

The present disclosure relates to a novel Bacillus megaterium HyangYak-01 strain and uses thereof. The novel Bacillus megaterium HyangYak-01 strain according to one aspect exhibits effects of increasing diversity of microbiome species in soil from crop fields or improving physiochemical properties, promoting crop growth or biosynthesis of biologically active substances, and the like, and thus can be applied for various uses, such as improving soil condition, increasing crop cultivation efficiency, or enhancing crop efficacy.