Bacillus sp. Strains for Plant Disease and Nematode Control

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Solution Overview

Problem

Current methods for controlling plant diseases and nematodes rely heavily on chemical pesticides, which pose environmental concerns and are becoming less effective due to resistance, while biological control methods lack stability and broad applicability.

Innovation Solution

Isolation of novel Bacillus sp. strains AT-332 and AT-79, which exhibit broad-spectrum antibacterial activity against plant diseases and nematodes, and promote plant growth, used as a microbiological agent for disease control and fertilizer.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical pesticides are used for controlling plant diseases and nematodes, then control effectiveness is improved, but environmental harm and drug resistance increase

Engineering Contradiction:

Improvecontrol effectivenessVSAvoidenvironmental harm

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the fundamental parameter of the control agent from chemical to biological origin. The Bacillus sp. AT-332 strain produces natural antimicrobial substances and antibiotics that control plant diseases and nematodes, replacing synthetic chemical pesticides while reducing environmental harm and resistance development

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent introduces a biological intermediary (Bacillus sp. AT-332 strain) that mediates between the plant and pathogens. The microorganism produces antimicrobial compounds and competes with pathogens for resources, providing control through biological interactions rather than direct chemical toxicity

Inventive Principle:

Principle #24Intermediary (Mediator)

2Object-affected harmful factors

If conventional microbiological pesticides are used, then environmental friendliness is improved, but control stability and applicability decrease

Engineering Contradiction:

Improveenvironmental friendlinessVSAvoidcontrol stability

Core Design Contradiction:

Object-affected harmful factorsVSReliability

Solution Approach 1:

The Bacillus sp. AT-332 strain exhibits multi-functionality by controlling diverse plant diseases (fungal, bacterial, viral) and nematode damage simultaneously. This broad-spectrum activity provides stable and reliable control across different pathogen types, overcoming the limited applicability of conventional single-target microbiological pesticides

Inventive Principle:

Principle #6Universality (Multi-functionality)

Solution Approach 2:

The patent utilizes the composite nature of the Bacillus sp. AT-332 strain, which produces multiple types of antimicrobial substances including antibiotics, lipopeptides, and other metabolites. This composite biological control mechanism provides stable efficacy against diverse pathogens through multiple modes of action

Inventive Principle:

Principle #40Composite materials

3Reliability

If single-function microbiological agents are used, then specific disease control is improved, but broad applicability decreases

Engineering Contradiction:

Improvedisease control effectivenessVSAvoidapplicable diseases range

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The Bacillus sp. AT-332 strain is engineered to perform multiple functions: controlling fungal diseases, bacterial diseases, viral diseases, and nematode damage simultaneously. This universal applicability resolves the contradiction by maintaining high effectiveness across diverse pathogen types rather than specializing in a single target

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The Bacillus sp. strains effectively control a wide range of plant diseases and nematodes, promoting plant growth and offering a stable, environmentally friendly alternative to chemical pesticides.

Implementation Method 1

The Bacillus sp. strains AT-332 and AT-79, which are a novel microorganism exhibiting much superior effects in controlling plant diseases and nematode damage

Methodology Applied

Scientific EffectAntimicrobial activity:

Implementation Method 2

promoting the growth of plants compared to microorganisms belonging to a closely-related Bacillus amyloliquefaciens disclosed in the literature

Methodology Applied

Scientific EffectPlant growth promotion:

Data Source

PatentUS10219517B2Strain belonging to Bacillus genus, microbiological agent, and plant cultivation method

Publication Date: 2019.03.05 SDS BIOTECH CO LTD

AI summary

The present invention relates to the Bacillus sp. strains AT-332 (NITE BP-1095) and AT-79 (NITE BP-1094) isolated from nature; and a plant disease control agent, a nematode control agent and a plant growth promoter containing the strains as active bacteria. The Bacillus sp. strains AT-332 and AT-79 strain are effective in controlling both a wide range of various plant diseases and nematode-damage and capable of promoting the growth of useful plants, due to a culture containing a secondary metabolite of the strains or cultivated and isolated live bacteria of the strains being introduced to a plant body or to the culture soil.