Bacillus subtilis DSM 32685 for Pathogen Inhibition and Polysaccharide Degradation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current Bacillus subtilis strains used in animal feed do not effectively inhibit the growth of multiple pathogenic bacteria such as Staphylococcus pseudintermedius, Streptococcus canis, E. coli, and Salmonella enterica, nor do they efficiently degrade non-starch polysaccharides to increase available oligosaccharides, which limits their ability to reduce feed energy content without compromising nutritional value.
Innovation Solution
The Bacillus subtilis strain DSM 32685, which is susceptible to antibiotics and specifically inhibits the growth of Staphylococcus pseudintermedius, Streptococcus canis, E. coli, and Salmonella enterica, and facilitates the degradation of non-starch polysaccharides, thereby increasing the amount of available oligosaccharides in animal feed, is used in animal feed formulations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional Bacillus subtilis strains are used in animal feed, then the feed maintains its basic nutritional function, but the strains fail to effectively inhibit pathogenic bacteria growth and degrade non-starch polysaccharides
Solution Approach 1:
The patent applies parameter changes by selecting and characterizing a specific Bacillus subtilis strain (DSM 32685) with optimized biological parameters including antibiotic susceptibility profile, enhanced pathogen inhibition zones, and superior NSP degradation capacity. This strain exhibits specific phenotypic parameters that differentiate it from conventional strains, enabling simultaneous achievement of reliable pathogen inhibition and high productivity in oligosaccharide production.
2Use of energy by moving object
If the energy content of feed is reduced, then the nutritional efficiency improves, but the ability to inhibit pathogenic bacteria and degrade NSP is compromised with conventional strains
Solution Approach 1:
The Bacillus subtilis DSM 32685 strain applies self-service by autonomously degrading non-starch polysaccharides in the feed to produce oligosaccharides, thereby actively reducing the feed's energy content while simultaneously maintaining its probiotic functionality. The strain serves itself by utilizing the NSP substrate present in the feed matrix, converting it into beneficial oligosaccharides without requiring external intervention, thus achieving both energy reduction and functional maintenance simultaneously.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
DSM 32685 effectively inhibits pathogenic bacteria growth and degrades non-starch polysaccharides, enhancing the nutritional value and reducing energy content of animal feed by increasing oligosaccharide availability, providing a superior alternative to existing strains.
Implementation Method 1
the Bacillus subtilis strain deposited as DSM 32685 and mutant strains thereof... which also have the ability to facilitate degradation of non-starch polysaccharides (NSP's) and thereby increase the amount of available oligosaccharides (sugar) in animal feed
Implementation Method 2
The present invention relates to the Bacillus subtilis strain deposited as DSM 32685 and mutant strains thereof which are susceptible to relevant antibiotics, inhibit growth of multiple Staphylococcus spp., Streptococcus canis, E. coli, and Salmonella enterica
Data Source
AI summary
Described are the Bacillus subtilis strain deposited as DSM 32685 and mutant strains thereof which are susceptible to relevant antibiotics, have inhibitory activity against, for example, Staphylococcus, Streptococcus, E. coli and Salmonella, and have the ability to facilitate degradation of non-starch polysaccharides and thereby increase the amount of available oligosaccharides (sugar) in animal feed. Also described are compositions comprising at least one Bacillus subtilis strain of the disclosure and optionally further bacteria and/or one or more types of yeast and methods of using such compositions.