Bacillus subtilis Insecticide Formulation with Palatability Enhancers

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Solution Overview

Problem

Conventional insecticides pose environmental risks and are not suitable for organic production, necessitating a broad-spectrum insecticidal formulation that effectively controls various insect pests without these limitations.

Innovation Solution

A formulation comprising Bacillus subtilis strain No. 5 NRRL 50391 spores, combined with palatability enhancers and endospore dispersion agents like polyamide, sorbitol, and diatomaceous earth, which is formulated into various forms such as suspensions, baits, and seed coatings to enhance insecticidal efficacy.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional insecticides are used for pest control, then insecticidal efficacy is achieved, but environmental harm increases

Engineering Contradiction:
Improveinsecticidal efficacyVSAvoidenvironmental harm
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the chemical composition parameters by replacing conventional synthetic insecticides with a biological formulation containing Bacillus subtilis spores, polyamide, sorbitol, ammonium oxalate, and sodium hexametaphosphate. This parameter change maintains insecticidal efficacy while eliminating environmental harm associated with conventional chemicals.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite biological insecticide formulation combining multiple components: Bacillus subtilis spores as the active agent, polyamide for spore dispersion, sorbitol as a carrier, ammonium oxalate and sodium hexametaphosphate as adjuvants. This composite material achieves effective pest control without the environmental harm of conventional insecticides.

Inventive Principle:
Principle #40Composite materials

2Object-affected harmful factors

If biological insecticides are used instead of conventional ones, then environmental harm is reduced, but insecticidal efficacy may be compromised

Engineering Contradiction:
Improveenvironmental harmVSAvoidinsecticidal efficacy
Core Design Contradiction:
Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent uses polyamide, sorbitol, ammonium oxalate, and sodium hexametaphosphate as intermediary substances that facilitate the delivery and activation of Bacillus subtilis spores. These intermediaries enhance spore dispersion, adhesion to plant surfaces, and penetration into insect pests, thereby ensuring reliable insecticidal efficacy while maintaining environmental safety.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent optimizes the concentration parameters of each component in the biological formulation to ensure effective pest control. By carefully adjusting the amounts of Bacillus subtilis spores, polyamide, sorbitol, ammonium oxalate, and sodium hexametaphosphate, the formulation achieves reliable insecticidal activity comparable to conventional insecticides while remaining environmentally safe.

Inventive Principle:
Principle #35Parameter changes

3Stability of the object's composition

If endospore dispersion enhancers are added to the formulation, then spore distribution is improved, but formulation complexity increases

Engineering Contradiction:
Improvespore distributionVSAvoidformulation complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent merges multiple functions into a single integrated formulation: Bacillus subtilis spores provide insecticidal activity, polyamide ensures spore dispersion and stability, sorbitol acts as a carrier and adjuvant, ammonium oxalate and sodium hexametaphosphate enhance spore activation and persistence. This merging of functions achieves effective spore distribution and reliable pest control through one unified product rather than multiple separate applications.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent creates a multi-functional biological insecticide formulation where each component serves multiple purposes: polyamide provides spore dispersion and formulation stability; sorbitol acts as carrier, adjuvant, and penetration enhancer; ammonium oxalate and sodium hexametaphosphate serve as activation agents and persistence enhancers. This multi-functionality reduces the need for separate additives and simplifies the overall formulation while achieving effective spore distribution.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The formulation effectively controls a wide range of insect pests, including grapevine leafhoppers, with significant efficacy demonstrated in laboratory assays, offering a sustainable alternative to conventional insecticides that does not harm the environment.

Implementation Method 1

The formulation allows the control of pests, without the need to use conventional insecticides

Methodology Applied
Scientific EffectAdhesion: Adhesive

Implementation Method 2

Bacillus subtilis strain No. 5 NRRL 50391, or the formulation further comprises spores of the bacterium Bacillus subtilis strain No. 5 NRRL 50391

Methodology Applied
Scientific EffectToxin release: Enzyme

Implementation Method 3

the formulation further comprises diatomaceous earth, whereby the exposure of insect epithelium to the endospores is increased

Methodology Applied
Scientific EffectMechanical exposure: Abrasion

Implementation Method 4

endospore dispersion enhancers, selected from the group consisting of polyamide, sorbitol, ammonium oxalate, sodium hexametaphosphate

Methodology Applied
Scientific EffectDispersion: Dispersion (of waves)

Implementation Method 5

As attractants (palatability enhancers), a protein source, and a carbohydrate source are included

Methodology Applied
Scientific EffectPalatability enhancement: Absorption (physical)

Data Source

PatentUS20240206476A1Biological insecticide formulation, free of chemical products, for insect pest control in plants
Publication Date: 2024.06.27 BIO INSUMOS NATIVA SPA

AI summary

Chemical-free, biological insecticide formulation for the control of insect pests in plants, wherein said formulation comprises Bacillus subtilis strain No. 5 NRRL 50391, at defined concentrations, palatability enhancers or attractants, a carrier selected from clays, kaolin, tale, zeolite, water, vegetable oils, paraffinic or non-paraffinic minerals among other agents.