Bacillus subtilis Composition for Mycotoxin Degradation
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Solution Overview
Problem
Current methods for eliminating mycotoxins from foodstuffs are either ineffective, lead to negative ecological and health impacts, or alter the nutritional value of products, and existing solutions are either unselective, too selective, or have limitations in degrading multiple mycotoxins simultaneously.
Innovation Solution
A composition comprising specific strains of Bacillus subtilis (NOL01, NOL02, and NOL03) is used to degrade multiple types of mycotoxins, effectively breaking down toxins without producing toxic residues, functioning across a wide range of pH, temperature, and conditions, including aerobic and anaerobic environments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If binders are used to eliminate mycotoxins, then mycotoxin removal is achieved, but binders become saturated by food matrices and interact with nutrients reducing their bioavailability
Solution Approach 1:
The patent replaces physical/chemical binders with a biological system (bacterial strains) that metabolically transform mycotoxins. Instead of binding mycotoxins through physical adsorption which competes with nutrient binding, the bacterial enzymes specifically metabolize mycotoxins into non-toxic compounds, eliminating the trade-off between mycotoxin removal and nutrient bioavailability.
Solution Approach 2:
The bacterial strains act as intermediary organisms that facilitate mycotoxin degradation through metabolic processes. The bacteria transform mycotoxins into non-toxic metabolites through enzymatic pathways, serving as a biological mediator that removes mycotoxins without directly interacting with or binding nutrients, thus preserving nutrient bioavailability.
2Measurement precision
If selective binders are used for specific mycotoxins, then targeted mycotoxin removal is achieved, but multiple mycotoxins require multiple solutions
Solution Approach 1:
The patent employs bacterial strains with broad-spectrum mycotoxin degradation capabilities. The bacterial system performs multiple functions by metabolizing various mycotoxin types (aflatoxins, ochratoxin A, zearalenone, deoxynivalenol, fumonisins) through different enzymatic pathways, replacing the need for multiple selective binders with a single universal biological solution.
Solution Approach 2:
The invention uses composite bacterial cultures comprising multiple strains (e.g., Bacillus subtilis, Lactobacillus, Saccharomyces) that work synergistically. Each strain contributes specific degradative enzymes targeting different mycotoxin classes, creating a composite biological system that handles diverse mycotoxins more effectively than individual strains alone.
3Object-affected harmful factors
If current mycotoxin elimination methods are used, then some mycotoxins are removed, but the nutritional value of products is altered
Solution Approach 1:
The patent replaces physical removal methods (washing, sorting, mechanical separation) and chemical treatments (ammonia treatment, thermal shock) with biological metabolism. The bacterial system selectively transforms mycotoxins into non-toxic compounds through enzymatic pathways that do not affect nutritional components, preserving product nutritional value while eliminating mycotoxins.
Solution Approach 2:
The bacterial system converts harmful mycotoxins into beneficial or neutral metabolites. Through metabolic pathways, the bacteria transform toxic compounds into non-toxic substances that can even serve as carbon sources or growth substrates, thereby converting the harmful mycotoxin presence into a beneficial biological process that preserves nutritional quality.
4Object-affected harmful factors
If ammonia treatment is used for detoxification, then mycotoxin removal is achieved, but negative ecological and health impacts occur
Solution Approach 1:
The patent replaces chemical ammonia treatment with biological bacterial metabolism. Instead of using ammonia under pressure which has ecological and health concerns, the system employs bacterial strains that naturally metabolize mycotoxins through enzymatic pathways, providing an eco-friendly and health-safe alternative that eliminates mycotoxins without generating harmful byproducts.
Solution Approach 2:
The bacterial strains are used as temporary, biodegradable agents for mycotoxin degradation. The bacteria complete their detoxification function and can be easily removed or degraded themselves, leaving no persistent chemical residues. This disposable biological approach replaces persistent chemical treatments with transient, environmentally benign biological agents.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition achieves nearly complete degradation of mycotoxins, making foodstuffs safe for consumption while preserving nutritional value, and can be used in various environments, including animal feed, to prevent mycotoxin-related pathologies.
Implementation Method 1
specific strains of bacteria of the genus Bacillus subtilis are capable of degrading several types of mycotoxins
Data Source
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AI summary
The invention relates to the use of a composition comprising at least one strain of Bacillus subtilis , for the degradation of mycotoxins.